Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

A guide to light-sheet fluorescence microscopy for multiscale imaging

RM Power, J Huisken - Nature methods, 2017 - nature.com
The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

Light-sheet microscopy: a tutorial

OE Olarte, J Andilla, EJ Gualda… - Advances in Optics and …, 2018 - opg.optica.org
This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

The light-sheet microscopy revolution

JM Girkin, MT Carvalho - Journal of Optics, 2018 - iopscience.iop.org
This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …

Application of vibrational spectroscopy and imaging to point-of-care medicine: a review

S Pahlow, K Weber, J Popp, BR Wood… - Applied …, 2018 - journals.sagepub.com
Vibrational spectroscopy and imaging promise molecular information that can be rapidly
acquired without the need for specialized stains or dyes, thereby potentially simplifying and …

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

S Bakthavatsalam, K Dodo, M Sodeoka - RSC Chemical Biology, 2021 - pubs.rsc.org
Alkyne functional groups have Raman signatures in a region (1800 cm− 1 to 2800 cm− 1)
that is free from interference from cell components, known as the “silent region”, and alkyne …

Volumetric chemical imaging by stimulated Raman projection microscopy and tomography

X Chen, C Zhang, P Lin, KC Huang, J Liang… - Nature …, 2017 - nature.com
Volumetric imaging allows global understanding of three-dimensional (3D) complex
systems. Light-sheet fluorescence microscopy and optical projection tomography have been …

Raman spectroscopy as a tool to study the pathophysiology of brain diseases

O Terrones, J Olazar-Intxausti, I Anso… - International Journal of …, 2023 - mdpi.com
The Raman phenomenon is based on the spontaneous inelastic scattering of light, which
depends on the molecular characteristics of the dispersant. Therefore, Raman spectroscopy …

More than double the fun with two-photon excitation microscopy

P Luu, SE Fraser, F Schneider - Communications Biology, 2024 - nature.com
For generations researchers have been observing the dynamic processes of life through the
lens of a microscope. This has offered tremendous insights into biological phenomena that …

Modular multimodal platform for classical and high throughput light sheet microscopy

M Bernardello, EJ Gualda, P Loza-Alvarez - Scientific reports, 2022 - nature.com
Light-sheet fluorescence microscopy (LSFM) has become an important tool for biological
and biomedical research. Although several illumination and detection strategies have been …