A rapid and accurate method for the estimation of protein concentration is essential in
various areas of biology and biochemistry. An assay originally described by Bradford (1) has …

Spectrophotometric determination of total protein is used in several areas such as clinical
analysis, food science and technology, biochemistry, protein chemistry, physiology. Five …

Publisher Summary This chapter discusses various methods of estimating protein
concentration. Absorption spectroscopy involves the absorption of a photon by an electron …

Scatchard analysis of binding of 125l‐basic fibroblast growth factor (FGF) to baby hamster
kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of …

The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a
plate reader to reduce the time needed for analyses. The total volume of the assay was …

Principles and Reactions of Protein Extraction, Purification, and Characterization provides
the mechanisms and experimental procedures for classic to cutting-edge techniques used in …

Human peritoneal mesothelial cells synthesize interleukin-6: Induction by IL-1β and TNFα.
Recent studies have demonstrated increased levels of IL-6 in the peritoneal cavity during …

Cystatin C, an efficient inhibitor of cysteine proteinases, is present in all investigated human
extracellular fluids. Dexamethasone caused a significant and dose-dependent increase in …

Polymethyl methacrylate (PMMA) films were treated by oxygen plasma discharge followed
by acrylic acid (AA) grafting. The amount of carboxyl groups introduced by the grafting of AA …

It has now been established that infection with human papillomavirus (HPV) is necessary for
the development of most cervical cancers. HPV is not sufficient for the development of …