As our understanding of the cell interior has grown, we have come to appreciate that most
cellular operations are localized, that is, they occur at discrete and identifiable locations or …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

Ribosomes catalyze protein synthesis by cycling through various functional states. These
states have been extensively characterized in vitro, but their distribution in actively …

In all species, ribosomes synthesize proteins by faithfully decoding messenger RNA (mRNA)
nucleotide sequences using aminoacyl-tRNA substrates. Current knowledge of the …

Advances in cryo-electron tomography (cryo-ET) have produced new opportunities to
visualize the structures of dynamic macromolecules in native cellular environments. While …

Cryo-electron tomography (cryo-ET) enables observation of macromolecular complexes in
their native, spatially contextualized cellular environment. Cryo-ET processing software to …

In situ cryo-electron tomography enables investigation of macromolecules in their native
cellular environment. Samples have become more readily available owing to recent …

Cryo-electron tomography provides 3D images of macromolecules in their cellular context.
To detect macromolecules in tomograms, template matching (TM) is often used, which uses …

Cryo–electron tomography (cryo-ET) is a powerful method to elucidate subcellular
architecture and to structurally analyze biomolecules in situ by subtomogram averaging, yet …