Protein analysis by shotgun/bottom-up proteomics
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
Recent advances (2019–2021) of capillary electrophoresis‐mass spectrometry for multilevel proteomics
Multilevel proteomics aims to delineate proteins at the peptide (bottom‐up proteomics),
proteoform (top‐down proteomics), and protein complex (native proteomics) levels. Capillary …
proteoform (top‐down proteomics), and protein complex (native proteomics) levels. Capillary …
Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis–mass …
We have reported a set of electrokinetically pumped sheath flow nanoelectrospray interfaces
to couple capillary zone electrophoresis with mass spectrometry. A separation capillary is …
to couple capillary zone electrophoresis with mass spectrometry. A separation capillary is …
[HTML][HTML] Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests
L Sun, G Zhu, Y Zhao, X Yan, S Mou… - … (International ed. in …, 2013 - ncbi.nlm.nih.gov
Femtogram proteomics: We report an ultrasensitive capillary zone electrophoresis-mass
spectrometry system based on an improved nanospray interface. This system is used for …
spectrometry system based on an improved nanospray interface. This system is used for …
Low-flow sheathless capillary electrophoresis–mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins
R Haselberg, GJ de Jong, GW Somsen - Analytical chemistry, 2013 - ACS Publications
Capillary electrophoresis coupled to time-of-flight mass spectrometry (CE–TOF-MS) via a
porous tip sheathless electrospray ionization (ESI) interface was studied for the …
porous tip sheathless electrospray ionization (ESI) interface was studied for the …
Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry
PB Pandeswari, V Sabareesh - RSC advances, 2019 - pubs.rsc.org
Owing to rapid growth in the elucidation of genome sequences of various organisms,
deducing proteome sequences has become imperative, in order to have an improved …
deducing proteome sequences has become imperative, in order to have an improved …
The coordinated action of the MVB pathway and autophagy ensures cell survival during starvation
The degradation and recycling of cellular components is essential for cell growth and
survival. Here we show how selective and non-selective lysosomal protein degradation …
survival. Here we show how selective and non-selective lysosomal protein degradation …
Capillary electrophoresis coupled to electrospray ionization tandem mass spectrometry for ultra-sensitive proteomic analysis of limited samples
KR Johnson, M Gregus, JC Kostas… - Analytical …, 2022 - ACS Publications
In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics
analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 …
analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 …
Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis–tandem mass spectrometry for proteomic analysis
Y Wang, BR Fonslow, CCL Wong… - Analytical …, 2012 - ACS Publications
We describe a solid phase microextraction (SPME), multistep elution, transient
isotachophoresis (tITP) capillary electrophoresis–tandem mass spectrometry (CE–MS/MS) …
isotachophoresis (tITP) capillary electrophoresis–tandem mass spectrometry (CE–MS/MS) …
Capillary electrophoresis–electrospray ionization-mass spectrometry interfaces: Fundamental concepts and technical developments
G Bonvin, J Schappler, S Rudaz - Journal of Chromatography A, 2012 - Elsevier
Capillary electrophoresis (CE) hyphenated to electrospray ionization (ESI) mass
spectrometry (MS) is a powerful tool for analyzing a wide variety of analytes in different …
spectrometry (MS) is a powerful tool for analyzing a wide variety of analytes in different …