Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Recent advances (2019–2021) of capillary electrophoresis‐mass spectrometry for multilevel proteomics

D Chen, EN McCool, Z Yang, X Shen… - Mass spectrometry …, 2023 - Wiley Online Library
Multilevel proteomics aims to delineate proteins at the peptide (bottom‐up proteomics),
proteoform (top‐down proteomics), and protein complex (native proteomics) levels. Capillary …

Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis–mass …

L Sun, G Zhu, Z Zhang, S Mou… - Journal of proteome …, 2015 - ACS Publications
We have reported a set of electrokinetically pumped sheath flow nanoelectrospray interfaces
to couple capillary zone electrophoresis with mass spectrometry. A separation capillary is …

[HTML][HTML] Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests

L Sun, G Zhu, Y Zhao, X Yan, S Mou… - … (International ed. in …, 2013 - ncbi.nlm.nih.gov
Femtogram proteomics: We report an ultrasensitive capillary zone electrophoresis-mass
spectrometry system based on an improved nanospray interface. This system is used for …

Low-flow sheathless capillary electrophoresis–mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins

R Haselberg, GJ de Jong, GW Somsen - Analytical chemistry, 2013 - ACS Publications
Capillary electrophoresis coupled to time-of-flight mass spectrometry (CE–TOF-MS) via a
porous tip sheathless electrospray ionization (ESI) interface was studied for the …

Middle-down approach: a choice to sequence and characterize proteins/proteomes by mass spectrometry

PB Pandeswari, V Sabareesh - RSC advances, 2019 - pubs.rsc.org
Owing to rapid growth in the elucidation of genome sequences of various organisms,
deducing proteome sequences has become imperative, in order to have an improved …

The coordinated action of the MVB pathway and autophagy ensures cell survival during starvation

M Müller, O Schmidt, M Angelova, K Faserl, S Weys… - Elife, 2015 - elifesciences.org
The degradation and recycling of cellular components is essential for cell growth and
survival. Here we show how selective and non-selective lysosomal protein degradation …

Capillary electrophoresis coupled to electrospray ionization tandem mass spectrometry for ultra-sensitive proteomic analysis of limited samples

KR Johnson, M Gregus, JC Kostas… - Analytical …, 2022 - ACS Publications
In this work, we developed an ultra-sensitive CE-MS/MS method for bottom-up proteomics
analysis of limited samples, down to sub-nanogram levels of total protein. Analysis of 880 …

Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis–tandem mass spectrometry for proteomic analysis

Y Wang, BR Fonslow, CCL Wong… - Analytical …, 2012 - ACS Publications
We describe a solid phase microextraction (SPME), multistep elution, transient
isotachophoresis (tITP) capillary electrophoresis–tandem mass spectrometry (CE–MS/MS) …

Capillary electrophoresis–electrospray ionization-mass spectrometry interfaces: Fundamental concepts and technical developments

G Bonvin, J Schappler, S Rudaz - Journal of Chromatography A, 2012 - Elsevier
Capillary electrophoresis (CE) hyphenated to electrospray ionization (ESI) mass
spectrometry (MS) is a powerful tool for analyzing a wide variety of analytes in different …