All soluble proteins populate conformational ensembles that together constitute the native
state. Their fluctuations in water are intrinsic thermodynamic phenomena, and the …

Protein kinases have very dynamic structures and their functionality strongly depends on
their dynamic state. Active kinases reveal a dynamic pattern with residues clustering into …

Protein kinases are dynamically regulated signaling proteins that act as switches in the cell
by phosphorylating target proteins. To establish a framework for analyzing linkages between …

The first protein kinase structure, solved in 1991, revealed the fold that is shared by all
members of the eukaryotic protein kinase superfamily and showed how the conserved …

Highlights•Screening efforts have revealed enzymes with over 100 promiscuous
activities.•High-throughput screening for promiscuous activities can be accomplished in …

It is well-established that dynamics are central to protein function; their importance is
implicitly acknowledged in the principles of the Monod, Wyman and Changeux model of …

Protein kinases have evolved in eukaryotes to be highly dynamic molecular switches that
regulate a plethora of biological processes. Two motifs, a dynamic activation segment and a …

Glycogen synthase kinase-3 (GSK-3) is a key regulator of many cellular signaling pathways.
Unlike most kinases, GSK-3 is controlled by inhibition rather than by specific activation. In …

It is difficult to imagine where the signaling community would be today without the Protein
Data Bank. This visionary resource, established in the 1970s, has been an essential partner …

Most proteins consist of multiple domains. How do linkers efficiently transfer information
between sites that are on different domains to activate the protein? Mere flexibility only …