The post-translational modification of proteins with ubiquitin plays a central role in nearly all
aspects of eukaryotic biology. Historically, studies have focused on the conjugation of …

Ubiquitin (Ub) chain formation by homologous to E6AP C-terminus (HECT)-family E3 ligases
regulates vast biology, yet the structural mechanisms remain unknown. We used chemistry …

The DNA damage repair regulatory protein RNF168, a monomeric RING-type E3 ligase, has
a crucial role in regulating cell fate and DNA repair by specific and efficient ubiquitination of …

Multiple neurodegenerative diseases are characterized by aberrant proteinaceous
accumulations of tau. Here, we report a RING-in-between-RING-type E3 ligase, TRIAD3A …

Parkin is an E3 ubiquitin ligase implicated in early-onset forms of Parkinson's disease. It
catalyzes a transthiolation reaction by accepting ubiquitin (Ub) from an E2 conjugating …

Abstract The RING-between-RING (RBR) E3 ubiquitin ligase family in humans comprises 14
members and is defined by a two-step catalytic mechanism in which ubiquitin is first …

RING-between RING (RBR)-type ubiquitin (Ub) ligases (E3s) such as Parkin receive Ub
from Ub-conjugating enzymes (E2s) in response to ligase activation. However, the specific …

Proteins with destabilizing N-terminal residues are degraded via K48-linked ubiquitination-
dependent N-degron pathway, during which the HECT-type E3 ligase Ufd4 can further …

The linear ubiquitin chain assembly complex synthesises linear Ub chains which constitute
a binding and activation platform for components of the TNF signalling pathway. One of the …

The raccoon dog (Nyctereutes procyonoides) is an invasive canid species native to East
Asia with several distinct characteristics. Here, we report a chromosome-scale genome of …