[HTML][HTML] Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials

AI Freitas, L Domingues, TQ Aguiar - Journal of Advanced Research, 2022 - Elsevier
Background The potential applications of protein-engineered functional materials are so
wide and exciting that the interest in these eco-friendly advanced materials will further …

Biomimetic and bioinspired silicifications: Recent advances for biomaterial design and applications

MAA Abdelhamid, SP Pack - Acta Biomaterialia, 2021 - Elsevier
The rational design and controllable synthesis of functional silica-based materials have
gained increased interest in a variety of biomedical and biotechnological applications due to …

[HTML][HTML] Biomedical applications of solid-binding peptides and proteins

N Alvisi, R de Vries - Materials today Bio, 2023 - Elsevier
Over the past decades, solid-binding peptides (SBPs) have found multiple applications in
materials science. In non-covalent surface modification strategies, solid-binding peptides …

Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

D Seevaratnam, F Ansah, Y Aniweh… - Analytical and …, 2022 - Springer
Bacillus stearothermophilus large fragment (BSTLF) DNA polymerase is reported, isolated
on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry …

Protein engineering of antibody fragments for pharmaceutical production

A Kuwahara, K Ikebukuro, R Asano - Applied Physics Reviews, 2023 - pubs.aip.org
Antibody fragments without the Fc region are attracting attention in the pharmaceutical
industry due to their high ability to penetrate solid tissues, cost-effective expression using …

Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi

M Shad, A Nazir, M Usman, MW Akhtar… - International Journal of …, 2024 - Elsevier
Alpha amylase belonging to starch hydrolyzing enzymes has significant contributions to
different industrial processes. The enzyme production through recombinant DNA technology …

[HTML][HTML] Bare silica as an alternative matrix for affinity purification/immobilization of His-tagged proteins

AI Freitas, L Domingues, TQ Aguiar - Separation and Purification …, 2022 - Elsevier
Abstract “Green” protein purification/immobilization processes based on low-cost, earth-
abundant, and eco-friendly affinity matrices are highly desirable. Unmodified silica matrices …

Engineered bacteria with genetic circuits accumulating nanomagnets as MRI contrast agents

M Yavuz, M Ütkür, EŞ Kehribar, E Yağız, EÜ Sarıtaş… - Small, 2022 - Wiley Online Library
The demand for highly efficient cancer diagnostic tools increases alongside the high cancer
incidence nowadays. Moreover, there is an imperative need for novel cancer treatment …

Silaffin-3-derived pentalysine cluster as a new fusion tag for one-step immobilization and purification of recombinant Bacillus subtilis catalase on bare silica particles

MAA Abdelhamid, AMA Meligy, KB Yeo, CS Lee… - International journal of …, 2020 - Elsevier
Bio-catalysis by enzymes on solid surfaces has been implemented in several practical
applications. However, the current methods for efficient enzyme immobilization with retained …

Engineering of biofilms with a glycosylation circuit for biomaterial applications

ES Kehribar, ME Isilak, EU Bozkurt, J Adamcik… - Biomaterials …, 2021 - pubs.rsc.org
Glycosylation is a crucial post-translational modification for a wide range of functionalities.
Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as …