Diagnosis of invasive fungal infections: challenges and recent developments

W Fang, J Wu, M Cheng, X Zhu, M Du, C Chen… - Journal of biomedical …, 2023 - Springer
Background The global burden of invasive fungal infections (IFIs) has shown an upsurge in
recent years due to the higher load of immunocompromised patients suffering from various …

Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

The UNITE database for molecular identification and taxonomic communication of fungi and other eukaryotes: sequences, taxa and classifications reconsidered

K Abarenkov, RH Nilsson, KH Larsson… - Nucleic Acids …, 2024 - academic.oup.com
Abstract UNITE (https://unite. ut. ee) is a web-based database and sequence management
environment for molecular identification of eukaryotes. It targets the nuclear ribosomal …

The numbers of fungi: Contributions from traditional taxonomic studies and challenges of metabarcoding

C Phukhamsakda, RH Nilsson, CS Bhunjun… - Fungal diversity, 2022 - Springer
The global diversity of fungi has been estimated using several different approaches. There is
somewhere between 2–11 million estimated species, but the number of formally described …

[HTML][HTML] Past, present, and future perspectives of environmental DNA (eDNA) metabarcoding: A systematic review in methods, monitoring, and applications of global …

KM Ruppert, RJ Kline, MS Rahman - Global Ecology and Conservation, 2019 - Elsevier
Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity
wherein samples are taken from the environment via water, sediment or air from which DNA …

[HTML][HTML] Airborne environmental DNA for terrestrial vertebrate community monitoring

C Lynggaard, MF Bertelsen, CV Jensen, MS Johnson… - Current Biology, 2022 - cell.com
Biodiversity monitoring at the community scale is a critical element of assessing and
studying species distributions, ecology, diversity, and movements, and it is key to …

Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

Microbiome composition and function in aquatic vertebrates: small organisms making big impacts on aquatic animal health

L Sehnal, E Brammer-Robbins… - Frontiers in …, 2021 - frontiersin.org
Aquatic ecosystems are under increasing stress from global anthropogenic and natural
changes, including climate change, eutrophication, ocean acidification, and pollution. In this …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Algorithm for post-clustering curation of DNA amplicon data yields reliable biodiversity estimates

TG Frøslev, R Kjøller, HH Bruun, R Ejrnæs… - Nature …, 2017 - nature.com
DNA metabarcoding is promising for cost-effective biodiversity monitoring, but reliable
diversity estimates are difficult to achieve and validate. Here we present and validate a …