Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Abstract The RNA-guided CRISPR–Cas9 nuclease from Streptococcus pyogenes (SpCas9)
has been widely repurposed for genome editing,,,. High-fidelity (SpCas9-HF1) and …

Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but
methods to delineate their genome-wide, off-target cleavage activities have been lacking …

CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the
functionality of genetic elements, creating genetically modified organisms as well as …

The microbial adaptive immune system CRISPR mediates defense against foreign genetic
elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize …

Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destruction as part
of a bacterial adaptive immune system mediated by clustered regularly interspaced short …

CRISPR–Cas9 RNA-guided nucleases are a transformative technology for biology, genetics
and medicine owing to the simplicity with which they can be programmed to cleave specific …

Few discoveries transform a discipline overnight, but biologists today can manipulate cells in
ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity …