Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

The investigation of biological processes by chemical methods, commonly referred to as
chemical biology, often requires chemical access to biologically relevant macromolecules …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

A split intein capable of protein trans-splicing is identified in a DnaE protein of the
cyanobacterium Synechocystis sp. strain PCC6803. The N-and C-terminal halves of DnaE …

▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme
(Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE …

We have studied the naturally split α subunit of the DNA polymerase III (DnaE) intein from
Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent …

Biophysical techniques such as fluorescence spectroscopy and nuclear magnetic
resonance (NMR) spectroscopy provide a window into the inner workings of proteins. These …

The discovery of inteins in the early 1990s opened the door to a wide variety of new
technologies. Early engineered inteins from various sources allowed the development of self …

Protein splicing, the protein equivalent of RNA splicing, is a newly discovered
posttranslational process that proceeds through a branched protein intermediate and …