Best practices for single-cell analysis across modalities

L Heumos, AC Schaar, C Lance, A Litinetskaya… - Nature Reviews …, 2023 - nature.com
Recent advances in single-cell technologies have enabled high-throughput molecular
profiling of cells across modalities and locations. Single-cell transcriptomics data can now …

A reference cell tree will serve science better than a reference cell atlas

S Domcke, J Shendure - Cell, 2023 - cell.com
Single-cell biology is facing a crisis of sorts. Vast numbers of single-cell molecular profiles
are being generated, clustered and annotated. However, this is overwhelmingly ad hoc, and …

Lung adenocarcinoma promotion by air pollutants

W Hill, EL Lim, CE Weeden, C Lee, M Augustine… - Nature, 2023 - nature.com
A complete understanding of how exposure to environmental substances promotes cancer
formation is lacking. More than 70 years ago, tumorigenesis was proposed to occur in a two …

A spatially resolved atlas of the human lung characterizes a gland-associated immune niche

E Madissoon, AJ Oliver, V Kleshchevnikov… - Nature Genetics, 2023 - nature.com
Single-cell transcriptomics has allowed unprecedented resolution of cell types/states in the
human lung, but their spatial context is less well defined. To (re) define tissue architecture of …

High-resolution single-cell atlas reveals diversity and plasticity of tissue-resident neutrophils in non-small cell lung cancer

S Salcher, G Sturm, L Horvath, G Untergasser… - Cancer cell, 2022 - cell.com
Non-small cell lung cancer (NSCLC) is characterized by molecular heterogeneity with
diverse immune cell infiltration patterns, which has been linked to therapy sensitivity and …

Active eosinophils regulate host defence and immune responses in colitis

A Gurtner, C Borrelli, I Gonzalez-Perez, K Bach, IE Acar… - Nature, 2023 - nature.com
In the past decade, single-cell transcriptomics has helped to uncover new cell types and
states and led to the construction of a cellular compendium of health and disease. Despite …

SEACells infers transcriptional and epigenomic cellular states from single-cell genomics data

S Persad, ZN Choo, C Dien, N Sohail, I Masilionis… - Nature …, 2023 - nature.com
Metacells are cell groupings derived from single-cell sequencing data that represent highly
granular, distinct cell states. Here we present single-cell aggregation of cell states …

A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates

P He, K Lim, D Sun, JP Pett, Q Jeng, K Polanski… - Cell, 2022 - cell.com
We present a multiomic cell atlas of human lung development that combines single-cell RNA
and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging …

Single-cell transcriptomics for the assessment of cardiac disease

AMA Miranda, V Janbandhu, H Maatz… - Nature Reviews …, 2023 - nature.com
Cardiovascular disease is the leading cause of death globally. An advanced understanding
of cardiovascular disease mechanisms is required to improve therapeutic strategies and …

Population-level integration of single-cell datasets enables multi-scale analysis across samples

C De Donno, S Hediyeh-Zadeh, AA Moinfar… - Nature …, 2023 - nature.com
The increasing generation of population-level single-cell atlases has the potential to link
sample metadata with cellular data. Constructing such references requires integration of …