Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …

Recent achievements in genomics have created an infrastructure of biological information.
The enormous success of genomics promptly induced a subsequent explosion in …

Enzymatic digestion for protein sequencing usually requires much time, and does not
always result in high sequence coverage. Here we report the use of aqueous microdroplets …

Kinetics of in-gel digestion of proteins by modified and native trypsins was studied by MALDI
TOF mass spectrometry using 18O-labeled peptides as internal standards. The effect of the …

Recently, microdroplet reactions have aroused much interest because the microdroplet
provides a unique medium where organic reactions could be accelerated by a factor of 103 …

An alternative method to enzymatic digestion for protein identification by mass spectrometry
has been developed that is based on chemical cleavage by formic acid. This method was …

Recent studies have shown that ultrafast enzymatic digestion of proteins can be achieved in
microdroplet within 250 μs. Further investigation of peptides resulting from microdroplet …

A novel method is proposed for rapid identification of viruses and other organisms that show
a low number of biomarkers, based on the construction of databases of organism-specific …

Protein glycosylation was explored by direct nanoESI MS and MS/MS analysis of ZIC-HILIC-
enriched proteolytic glycopeptides without further separation or purification. In a previous …

Mutations in the central region of the signalling hub Adenomatous Polyposis Coli (APC)
cause colorectal tumourigenesis. The structure of this region remained unknown. Here, we …