Codon usage and tRNA abundance are critical parameters for gene synthesis. However, the
forces determining codon usage bias within genomes and between organisms, as well as …

The physical principles of DNA hybridization and folding are described within the context of
how they are important for designing optimal PCRs. The multi-state equilibrium model for …

There is a need for methods that can image chromosomes with genome-wide coverage, as
well as greater genomic and optical resolution. We introduce OligoFISSEQ, a suite of three …

DNA-encoded libraries have emerged as a widely used resource for the discovery of
bioactive small molecules, and offer substantial advantages compared with conventional …

The efficiency of DNA-enrichment techniques is often insufficient to detect mutations that
occur at low frequencies. Here we report a DNA-excision method for the detection of low …

The polymerase chain reaction is fundamental to molecular biology and is the most
important practical molecular technique for the research laboratory. We have developed and …

(*) Netleei subJeet to any dlselalmeri the term ofthls Pelicic, v. et al,(2000) J. Bacteriol. 182:
5391-5398. patent is extended or adjusted under 35 Yao, M. AndKow, YW.(1995) J. Biol …

CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While
the rigid identification of DNA sequences by these systems minimizes the potential for off …

Successful PCR starts with proper priming between an oligonucleotide primer and the
template DNA. However, the inevitable risk of mismatched priming cannot be avoided in the …

Thermophilic microorganisms are of special interest for phylogenetics and research in
prokaryotic evolution, since many of them belong to deep branches of the tree of life. For this …