Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications

AP Cheng, S Kwon, T Adeshara, V Göhre… - Applied microbiology …, 2023 - Springer
Extracellular RNAs are an emerging research topic in fungal-plant interactions. Fungal plant
pathogens and symbionts release small RNAs that enter host cells to manipulate plant …

Challenges in characterization of transcriptomes of extracellular vesicles and non-vesicular extracellular RNA carriers

J Makarova, D Maltseva, A Tonevitsky - Frontiers in Molecular …, 2023 - frontiersin.org
Since its original discovery over a decade ago, extracellular RNA (exRNA) has been found
in all biological fluids. Furthermore, extracellular microRNA has been shown to be involved …

Integrating extracellular vesicle and circulating cell‐free DNA analysis using a single plasma aliquot improves the detection of HER2 positivity in breast cancer …

V Mugoni, Y Ciani, O Quaini, S Tomasini… - Journal of …, 2023 - Wiley Online Library
Multi‐analyte liquid biopsies represent an emerging opportunity for non‐invasive cancer
assessment. We developed ONCE (One Aliquot for Circulating Elements), an approach for …

International Society for Extracellular Vesicles workshop. QuantitatEVs: Multiscale analyses, from bulk to single extracellular vesicle

M Basso, A Gori, C Nardella, M Palviainen, M Holcar… - 2024 - Wiley Online Library
The “QuantitatEVs: multiscale analyses, from bulk to single vesicle” workshop aimed to
discuss quantitative strategies and harmonized wet and computational approaches toward …

Comparison of EV characterization by commercial high‐sensitivity flow cytometers and a custom single‐molecule flow cytometer

J Kim, S Xu, SR Jung, A Nguyen… - Journal of …, 2024 - Wiley Online Library
High‐sensitivity flow cytometers have been developed for multi‐parameter characterization
of single extracellular vesicles (EVs), but performance varies among instruments and …

[HTML][HTML] Ribonuclease activity undermines immune sensing of naked extracellular RNA

M Castellano, V Blanco, ML Calzi, B Costa, K Witwer… - bioRxiv, 2024 - ncbi.nlm.nih.gov
The plasma membrane and the membrane of endosomal vesicles are considered physical
barriers preventing extracellular RNA uptake. While naked RNA can be spontaneously …

Intact messenger RNA exists in human blood plasma and urine, and their purified macromolecular compartments

J Verwilt, K Verniers, S De Geyter, S Roelandt… - bioRxiv, 2024 - biorxiv.org
It is generally assumed that extracellular long RNA molecules in biofluids are fragmented.
Few studies have indirectly hinted at the existence of possibly functional, intact long RNA …

Seminar: extracellular vesicles as mediators of environmental stress in human disease

V Kalia, AA Baccarelli, C Happel… - Environmental …, 2023 - ehp.niehs.nih.gov
Background: Extracellular vesicles (EVs), membrane-bound particles containing a variety of
RNA types, DNA, proteins, and other macromolecules, are now appreciated as an important …

The RNA-Recognition Pathway: An Overlooked Transportation Mechanism for Extracellular and Therapeutic RNAs

A Ghidini, A Singh - GEN Biotechnology, 2023 - liebertpub.com
In the central dogma proposed by Francis Crick in 1958, RNA simply plays an intermediary
role between DNA and protein expression. Over the last few decades, our understanding of …

[PDF][PDF] Lipoproteins as overlooked RNA carriers in saliva: implications for extracellular vesicle biomarker studies

TD Veit, F Visioli, JAB Chies - 2024 - pdf.elspublishing.com
Extracellular vesicles (EVs) are nanoscopic structures released by all cell types, playing a
crucial role in cellular communication. EVs contain various types of RNAs, and a significant …