Aptamers 101: aptamer discovery and in vitro applications in biosensors and separations
Aptamers are single-stranded nucleic acids that bind and recognize targets much like
antibodies. Recently, aptamers have garnered increased interest due to their unique …
antibodies. Recently, aptamers have garnered increased interest due to their unique …
High-throughput sequencing and metagenomics: moving forward in the culture-independent analysis of food microbial ecology
D Ercolini - Applied and environmental microbiology, 2013 - Am Soc Microbiol
Following recent trends in environmental microbiology, food microbiology has benefited
from the advances in molecular biology and adopted novel strategies to detect, identify, and …
from the advances in molecular biology and adopted novel strategies to detect, identify, and …
Ribosomal ITS sequences and plant phylogenetic inference
One of the most popular sequences for phylogenetic inference at the generic and
infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S–5.8 S …
infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S–5.8 S …
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
G Muyzer, K Smalla - Antonie van Leeuwenhoek, 1998 - Springer
Here, the state of the art of the application of denaturing gradient gel electrophoresis
(DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be …
(DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be …
PCR-DGGE fingerprinting: novel strategies for detection of microbes in food
D Ercolini - Journal of microbiological methods, 2004 - Elsevier
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE)
fingerprinting was recently introduced into food microbiology. This paper describes the …
fingerprinting was recently introduced into food microbiology. This paper describes the …
Direct RNA sequencing
F Ozsolak, AR Platt, DR Jones, JG Reifenberger… - Nature, 2009 - nature.com
Our understanding of human biology and disease is ultimately dependent on a complete
understanding of the genome and its functions. The recent application of microarray and …
understanding of the genome and its functions. The recent application of microarray and …
Guidelines for the tetra-primer ARMS–PCR technique development
RFV Medrano, CA De Oliveira - Molecular biotechnology, 2014 - Springer
The tetra-primer amplification refractory mutation system–polymerase chain (ARMS–PCR)
reaction is a simple and economical method to genotype single-nucleotide polymorphisms …
reaction is a simple and economical method to genotype single-nucleotide polymorphisms …
Pre-PCR processing: strategies to generate PCR-compatible samples
P Rådström, R Knutsson, P Wolffs, M Lövenklev… - Molecular …, 2004 - Springer
Polymerase chain reaction (PCR) is recognized as a rapid, sensitive, and specific molecular
diagnostic tool for the analysis of nucleic acids. However, the sensitivity and kinetics of …
diagnostic tool for the analysis of nucleic acids. However, the sensitivity and kinetics of …
Approaches to DNA mutagenesis: an overview
MM Ling, BH Robinson - Analytical biochemistry, 1997 - Elsevier
Mutagenesis of proteins is of paramount importance chain reaction (PCR) or non-PCR, has
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …
The evolution of ribosomal DNA divergent paralogues and phylogenetic implications
ES Buckler IV, A Ippolito, TP Holtsford - Genetics, 1997 - academic.oup.com
Although nuclear ribosomal DNA (rDNA) repeats evolve together through concerted
evolution, some genomes contain a considerable diversity of paralogous rDNA. This …
evolution, some genomes contain a considerable diversity of paralogous rDNA. This …