Targeting ultrafast spectroscopic insights into red fluorescent proteins

TD Krueger, C Chen, C Fang - Chemistry–An Asian Journal, 2023 - Wiley Online Library
Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …

Fluorescent proteins generate a genetic color polymorphism and counteract oxidative stress in intertidal sea anemones

DN Clarke, NH Rose… - Proceedings of the …, 2024 - National Acad Sciences
Fluorescent proteins (FPs) are ubiquitous tools in research, yet their endogenous functions
in nature are poorly understood. In this work, we describe a combination of functions for FPs …

Photophysical engineering of fluorescent proteins: Accomplishments and challenges of physical chemistry strategies

S Mukherjee, R Jimenez - The Journal of Physical Chemistry B, 2022 - ACS Publications
Fluorescent proteins (FPs) have become ubiquitous tools for biological research and
concomitantly they are intriguing molecules that are amenable to study with a wide range of …

To twist or not to twist: From chromophore structure to dynamics inside engineered photoconvertible and photoswitchable fluorescent proteins

TD Krueger, L Tang, C Chen, L Zhu, IL Breen… - Protein …, 2023 - Wiley Online Library
Green‐to‐red photoconvertible fluorescent proteins (FPs) are vital biomimetic tools for
powerful techniques such as super‐resolution imaging. A unique Kaede‐type FP named the …

Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins

TD Krueger, JN Henderson, IL Breen, L Zhu… - Frontiers in …, 2023 - frontiersin.org
Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes
for sophisticated and advanced bioimaging techniques. Among them, an interplay between …

Chromophore reduction plus reversible photobleaching: how the mKate2 “photoconversion” works

EA Protasova, AS Mishin, KA Lukyanov… - Photochemical & …, 2021 - Springer
Abstract mKate red-to-green photoconversion is a non-canonical type of
phototransformation in fluorescent proteins, with a poorly understood mechanism. We have …

Effect of the surrounding environment on electron beam irradiation damage of enhanced green fluorescent protein

H Osakabe, M Suzuki, T Shimizu, H Minoda - Ultramicroscopy, 2025 - Elsevier
Fluorescent proteins exhibit fluorescence and photoconversion, which are used to study
biological phenomena. Among these, enhanced green fluorescent protein (EGFP) emits …

Two-photon lifetime-based photoconversion of EGFP for 3D-photostimulation in FLIM

D Strachotová, A Holoubek, B Brodská… - … and Applications in …, 2023 - iopscience.iop.org
Enhanced green fluorescence protein (EGFP) is a fluorescent tag commonly used in cellular
and biomedical applications. Surprisingly, some interesting photochemical properties of …

Elucidating Contributions from Multiple Species during Photoconversion of Enhanced Green Fluorescent Protein (EGFP) under Ultraviolet Illumination

S Dhamija, AK De - Photochemistry and Photobiology, 2021 - Wiley Online Library
Photocycle in wild‐type green fluorescent protein (wt‐GFP) involves generation of a bright
fluorescent deprotonated chromophore from feebly fluorescent protonated form via excited …

Protein Mobility Measurements through Oxidative Green-to-Red Photoconversion of EGFP

VE Bochenkov, NS Sopova, AM Shakhov… - The Journal of …, 2022 - ACS Publications
Protein dynamics plays a key role in live cell functioning, stimulating the development of new
experimental techniques for studying protein transport phenomena. Here, we introduce a …