Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications

H Leemhuis, RM Kelly, L Dijkhuizen - Applied microbiology and …, 2010 - Springer
Cyclodextrin glucanotransferases (CGTases) are industrially important enzymes that
produce cyclic α-(1, 4)-linked oligosaccharides (cyclodextrins) from starch. Cyclodextrin …

Comprehensive study on transglycosylation of CGTase from various sources

CH Lim, B Rasti, J Sulistyo, MA Hamid - Heliyon, 2021 - cell.com
Transglycosylation is the in-vivo or in-vitro process of transferring glycosyl groups from a
donor to an acceptor, which is usually performed by enzymatic reactions because of their …

Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications

R Han, J Li, H Shin, RR Chen, G Du, L Liu… - Biotechnology Advances, 2014 - Elsevier
Cyclodextrin glycosyltransferase (CGTase) is an important enzyme with multiple functions, in
particular the production of cyclodextrins. It is also widely applied in baking and …

Glucosylation of flavonol and flavanones by Bacillus cyclodextrin glucosyltransferase to enhance their solubility and stability

YS Lee, JB Woo, SI Ryu, SK Moon, NS Han, SB Lee - Food Chemistry, 2017 - Elsevier
Enzymatically modified isoquercitrin (EMIQ), oligoglucosyl naringenin-7-(glucose [G]), and
oligoglucosyl hesperetin (H)-7-G were produced via oligoglucosylation of quercetin-3 …

Enzymatic modification enhances the protective activity of citrus flavonoids against alcohol-induced liver disease

HY Park, HD Choi, H Eom, I Choi - Food chemistry, 2013 - Elsevier
Alcoholic liver disease (ALD) can be developed by a prolonged or large intake of alcohol in
a short period of time. ALD is considered as a leading cause for a liver injury in modern …

Surface engineering of cyclodextrin glycosyltransferase reveals structural compactness and rigidity responsible for enhanced organic solvents resistance

R Han, Y Jiang, S Liu, Y Ji, U Schwaneberg, Y Ni - Molecular Catalysis, 2023 - Elsevier
Cyclodextrin glycosyltransferase (CGTase) is a preferable biocatalyst for production of
cyclodextrin and phenols glycosylated derivatives due to its cost-effective donors and broad …

Stability of Enzyme-Modified Flavonoid C- and O-Glycosides from Common Buckwheat Sprout Extracts during In Vitro Digestion and Colonic Fermentation

D Jang, YS Jung, H Seong, MS Kim… - Journal of Agricultural …, 2021 - ACS Publications
Common buckwheat sprout (CBS) contains more flavone C-glycosides (FCGs) and flavonol
O-glycosides (FOGs) than does common buckwheat seed. Both flavonoids in CBS are well …

Enzymatic synthesis, structural analysis, and evaluation of antibacterial activity and α-glucosidase inhibition of hesperidin glycosides

T Chaisin, P Rudeekulthamrong, J Kaulpiboon - Catalysts, 2021 - mdpi.com
This study was designed to investigate the structure of synthesized hesperidin glycosides
(HGs) and evaluate their antibacterial and α-glucosidase inhibitory activities. The …

Enhanced production of α-cyclodextrin glycosyltransferase in Escherichia coli by systematic codon usage optimization

H Liu, J Li, G Du, J Zhou, J Chen - Journal of Industrial …, 2012 - academic.oup.com
Enhancing the production of α-cyclodextrin glycosyltransferase (α-CGTase) is a key aim in α-
CGTase industries. Here, the mature α-cgt gene from Paenibacillus macerans JFB05-01 …

Engineering of cyclodextrin glycosyltransferase reveals pH-regulated mechanism of enhanced long-chain glycosylated sophoricoside specificity

R Han, J Ni, J Zhou, J Dong, G Xu… - Applied and …, 2020 - Am Soc Microbiol
Sophoricoside glycosylated derivatives, especially long-chain glycosylated sophoricosides
(LCGS), have greatly improved water solubility compared with sophoricoside. Here …