Molecular docking and molecular dynamics simulations of a mutant Acinetobacter haemolyticus alkaline-stable lipase against tributyrin

NFSK Anuar, RA Wahab, F Huyop… - Journal of …, 2021 - Taylor & Francis
We previously reported on a mutant lipase KV1 (Mut-LipKV1) from Acinetobacter
haemolyticus which optimal pH was raised from 8.0 to 11.0 after triple substitutions of …

Overexpression and Mutation of a Novel Lipase from Serratia marcescens L1 in Escherichia coli

H Chen, F Yu, N Shi, P Du, S Liu, X Zhang, J Tan - Process Biochemistry, 2021 - Elsevier
A novel lipase gene (lipA) from Serratia marcescens L1 was cloned and expressed in
Escherichia coli. LipA was shown to consist of 614 amino acid residues with seven residues …

In silico mutation on a mutant lipase from Acinetobacter haemolyticus towards enhancing alkaline stability

NFSK Anuar, RA Wahab, F Huyop… - Journal of …, 2020 - Taylor & Francis
Alkaline-stable lipases are highly valuable biocatalysts that catalyze reactions under highly
basic conditions. Herein, computational predictions of lipase from Acinetobacter …

Combination of simultaneous extraction–hydrolysis and intermittent feeding of tara pod for efficient production of gallic acid

X Tang, CL Liu, YY Chen, Y Wei, XY Zhuang… - Biochemical …, 2022 - Elsevier
The pods of Caesalpinia spinosa consists of gallotannins, which represent more than 50%
of its weight. The traditional chemical procedure used to produce gallic acid (GA) from tara …

Characterization of a carboxylesterase with hyper-thermostability and alkali-stability from Streptomyces lividans TK24

X Chang, S Wu, J Chen, S Xiong, P Wang, X Shi… - Extremophiles, 2021 - Springer
Abstract A gene (estA', 804 bp) from Streptomyces lividans TK24 was artificially synthesized
and successfully overexpressed as a 6His-tagged fusion protein in Escherichia coli. It …

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

EK Court, RR Chaudhuri, RV Kapoore… - …, 2021 - microbiologyresearch.org
Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils
and grease (FOG) present a major problem for sewer management. Their accumulation can …