Photoswitchable fluorescent proteins: mechanisms on ultrafast timescales

L Tang, C Fang - International Journal of Molecular Sciences, 2022 - mdpi.com
The advancement of super-resolution imaging (SRI) relies on fluorescent proteins with novel
photochromic properties. Using light, the reversibly switchable fluorescent proteins (RSFPs) …

Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging

K Mishra, JP Fuenzalida-Werner, F Pennacchietti… - Nature …, 2022 - nature.com
Reversibly photo-switchable proteins are essential for many super-resolution fluorescence
microscopic and optoacoustic imaging methods. However, they have yet to be used as …

Stimuli‐responsive natural proteins and their applications

N Du, F Ye, J Sun, K Liu - ChemBioChem, 2022 - Wiley Online Library
Natural proteins are essential biomacromolecules that fulfill versatile functions in the living
organism, such as their usage as cytoskeleton, nutriment transporter, homeostasis …

Reaction coordinates and transition states in enzymatic catalysis

K Zinovjev, I Tuñón - Wiley Interdisciplinary Reviews …, 2018 - Wiley Online Library
Enzymatic reactions are complex chemical processes taking place in complex dynamic
environments. Theoretical characterization of these reactions requires the determination of …

Structure–function dataset reveals environment effects within a fluorescent protein model system

E De Zitter, S Hugelier, S Duwé… - Angewandte …, 2021 - Wiley Online Library
Anisotropic environments can drastically alter the spectroscopy and photochemistry of
molecules, leading to complex structure‐function relationships. We examined this using …

Reversibly switchable fluorescent proteins:“the fair switch project”

R Nifosì, B Storti, R Bizzarri - La Rivista del Nuovo Cimento, 2024 - Springer
Fluorescent proteins (FPs) have transformed cell biology through their use in fluorescence
microscopy, enabling precise labeling of proteins via genetic fusion. A key advancement is …

Systematic excited state studies of reversibly switchable fluorescent proteins

D Smyrnova, MC Marin, M Olivucci… - Journal of Chemical …, 2018 - ACS Publications
The reversibly switchable fluorescent proteins Dronpa, rsFastLime, rsKame, Padron, and
bsDronpa feature the same chromophore but display a 40 nm variation in absorption …

Fluorescent proteins

MG Khrenova, AP Savitsky - Theoretical and Computational Photochemistry, 2023 - Elsevier
This chapter covers molecular modeling methods utilized to study the main photophysical
and photochemical events in fluorescent proteins. Among them, GFP-like proteins are the …

Integrated structure-function dataset reveals key mechanisms underlying photochromic fluorescent proteins

E De Zitter, S Hugelier, S Duwé, W Vandenberg… - bioRxiv, 2020 - biorxiv.org
Photochromic fluorescent proteins have become versatile tools in the life sciences, though
our understanding of their structure-function relation is limited. Starting from a single …

[PDF][PDF] Integrated structure-function dataset reveals insights into the mechanisms underlying reversibly switchable fluorescent proteins

E De Zitter, S Hugelier, S Duwé, W Vandenberg… - 2020 - lirias.kuleuven.be
Photochromic fluorescent proteins have become essential tools in the life sciences, though
our understanding of their structure-function relation is limited. Starting from a single …