1Progress, applications, challenges and prospects of protein purification technology

M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …

A cleavable self-aggregating tag scheme for the expression and purification of disulfide bonded proteins and peptides

Z Lin, Y Jing, Y Huang, S Yang, S Chen, Y Ou… - Chemical Engineering …, 2022 - Elsevier
The production of correctly folded, disulfide bonded proteins in high yields remains a
technological challenge, especially for the commonly used expression system Escherichia …

Maximizing the recovery of the native p28 bacterial peptide with improved activity and maintained solubility and stability in Escherichia coli BL21 (DE3)

H Abuei, M Pirouzfar, A Mojiri… - Journal of …, 2022 - Elsevier
Abstract p28 is a natural bacterial product, which recently has attracted much attention as an
efficient cell penetrating peptide (CPP) and a promising anticancer agent. Considering the …

Optimization of 2, 6-dimethoxy benzoquinone production through wheat germ fermentation by saccharomyces cerevisiae

M Parsazad, V Babaeipour… - Applied Food …, 2020 - journals.sbmu.ac.ir
Background and objective: Nowadays, anticancer effects of 2, 6 dimethoxy benzoquinone
are verified. Optimization of 2, 6 dimethoxy benzoquinone content of fermented wheat germ …

A Screening Model of Antibacterial Agents Based on Escherichia coli Cell-Division Protein

Q Fan, J Wu, B Xi, C Li, X Wang, H Li - Applied Sciences, 2023 - mdpi.com
Pathogenic Escherichia coli cannot be killed by most antibiotics (including colistin, a last-
resort drug) due to the rapid development of antibiotic resistance. A highly conserved …

Soluble overexpression, high-level production and purification of receptor binding domain of human VEGF8-109 in E. coli

S Rezaei, Z Takalloo, ZS Rezaei, V Babaeipour… - Process …, 2020 - Elsevier
The high-yield production of vascular endothelial growth factor (VEGF), as a major
therapeutic target in pathological angiogenesis and diabetic wound healing, provides critical …

A Taguchi approach for optimizing the expression of recombinant human growth hormone in Escherichia coli

E Moein Jahromi, AA Deldar, M Shahali - Microbiology, Metabolites …, 2021 - armmt.irost.ir
Expression of recombinant human growth hormone in E. coli requires specific measures to
be more efficient. In this study, two strains of E. coli, one with a genomic expression of T7 …

Growth hormone receptor agonists and antagonists: From protein expression and purification to long‐acting formulations

Y Wang, M Kim, C Buckley, HD Maynard… - Protein …, 2023 - Wiley Online Library
Recombinant human growth hormone (rhGH) and GH receptor antagonists (GHAs) are used
clinically to treat a range of disorders associated with GH deficiency or hypersecretion …

Diseño y optimización de proteínas terapéuticas

I Fernández Cisneros - 2021 - repositorio.unican.es
En la actualidad las proteínas terapéuticas son fármacos fundamentales que nos han
permitido mejorar el tratamiento de un gran número de patologías, el desarrollo de la …

[引用][C] Exploring Downstream Processing Strategies for Active Pharmaceutical Ingredients (APIs) Derived from Industrial Fermentation

AA Nadeem, A Hussain, M Shaukat, S Nazir, T Anwar…