A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Unravelling interspecies interactions across heterogeneities in complex biofilm communities
HL Røder, NMC Olsen, M Whiteley… - Environmental …, 2020 - Wiley Online Library
The importance of microbial biofilms has been well‐recognized for several decades, and
focus is now shifting towards investigating multispecies biofilm communities rather than …
focus is now shifting towards investigating multispecies biofilm communities rather than …
DEG 15, an update of the Database of Essential Genes that includes built-in analysis tools
Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
The impact of genetic diversity on gene essentiality within the Escherichia coli species
F Rousset, J Cabezas-Caballero, F Piastra-Facon… - Nature …, 2021 - nature.com
Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …
the set of genes shared by all strains, known as the core genome, represents about half the …
In situ targeted base editing of bacteria in the mouse gut
AK Brödel, LH Charpenay, M Galtier, FJ Fuche… - Nature, 2024 - nature.com
Microbiome research is now demonstrating a growing number of bacterial strains and genes
that affect our health. Although CRISPR-derived tools have shown great success in editing …
that affect our health. Although CRISPR-derived tools have shown great success in editing …
Nutrient stress is a target for new antibiotics
LA Carfrae, ED Brown - Trends in microbiology, 2023 - cell.com
Novel approaches are required to address the looming threat of pan-resistant Gram-
negative pathogens and forestall the rise of untreatable infections. Unconventional targets …
negative pathogens and forestall the rise of untreatable infections. Unconventional targets …
[HTML][HTML] Enterohemorrhagic Escherichia coli senses microbiota-derived nicotinamide to increase its virulence and colonization in the large intestine
W Yang, H Sun, J Yan, C Kang, J Wu, B Yang - Cell Reports, 2023 - cell.com
Summary Enterohemorrhagic Escherichia coli (EHEC) O157: H7 is a foodborne pathogen
that specifically colonizes and infects the human large intestine. EHEC O157: H7 engages …
that specifically colonizes and infects the human large intestine. EHEC O157: H7 engages …
Mechanisms of Virulence Reprogramming in Bacterial Pathogens
Bacteria are single-celled organisms that carry a comparatively small set of genetic
information, typically consisting of a few thousand genes that can be selectively activated or …
information, typically consisting of a few thousand genes that can be selectively activated or …
Pathogen clonal expansion underlies multiorgan dissemination and organ-specific outcomes during murine systemic infection
K Hullahalli, MK Waldor - Elife, 2021 - elifesciences.org
The dissemination of pathogens through blood and their establishment within organs lead to
severe clinical outcomes. However, the within-host dynamics that underlie pathogen spread …
severe clinical outcomes. However, the within-host dynamics that underlie pathogen spread …
Transposon insertion sequencing, a global measure of gene function
T van Opijnen, HL Levin - Annual review of genetics, 2020 - annualreviews.org
The goal of genomics and systems biology is to understand how complex systems of factors
assemble into pathways and structures that combine to form living organisms. Great …
assemble into pathways and structures that combine to form living organisms. Great …