Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Bacteria utilise specialised protein secretion systems to interact with host organisms,
competitor bacteria, and the environment. The Type VI secretion system (T6SS) is a versatile …

Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Understanding the relations between the formation, structure, dynamics and functionality of
complex synthetic materials is one of the great challenges in chemistry and nanotechnology …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …