Methods to evaluate bacterial motility and its role in bacterial–host interactions

V Palma, MS Gutiérrez, O Vargas, R Parthasarathy… - Microorganisms, 2022 - mdpi.com
Bacterial motility is a widespread characteristic that can provide several advantages for the
cell, allowing it to move towards more favorable conditions and enabling host-associated …

Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning

Z Wang, L Zhu, H Zhang, G Li, C Yi, Y Li, Y Yang… - Nature …, 2021 - nature.com
Light-field microscopy has emerged as a technique of choice for high-speed volumetric
imaging of fast biological processes. However, artifacts, nonuniform resolution and a slow …

Quantity as a fish views it: Behavior and neurobiology

A Messina, D Potrich, M Perrino… - Frontiers in …, 2022 - frontiersin.org
An ability to estimate quantities, such as the number of conspecifics or the size of a predator,
has been reported in vertebrates. Fish, in particular zebrafish, may be instrumental in …

Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy

Z Zhang, L Bai, L Cong, P Yu, T Zhang, W Shi… - Nature …, 2021 - nature.com
A detailed understanding of the function of neural networks and how they are supported by a
dynamic vascular system requires fast three-dimensional imaging in thick tissues. Here we …

Deep learning-enhanced light-field imaging with continuous validation

N Wagner, F Beuttenmueller, N Norlin, J Gierten… - Nature …, 2021 - nature.com
Visualizing dynamic processes over large, three-dimensional fields of view at high speed is
essential for many applications in the life sciences. Light-field microscopy (LFM) has …

Neurophotonic tools for microscopic measurements and manipulation: status report

AS Abdelfattah, S Ahuja, T Akkin, SR Allu… - …, 2022 - spiedigitallibrary.org
Neurophotonics was launched in 2014 coinciding with the launch of the BRAIN Initiative
focused on development of technologies for advancement of neuroscience. For the last …

High-contrast multifocus microscopy with a single camera and z-splitter prism

S Xiao, H Gritton, H Tseng, D Zemel, X Han, J Mertz - Optica, 2020 - opg.optica.org
Optical microscopy has been an indispensable tool for studying complex biological systems,
but is often hampered by problems of speed and complexity when performing 3D volumetric …

A practical guide to scanning light-field microscopy with digital adaptive optics

Z Lu, Y Cai, Y Nie, Y Yang, J Wu, Q Dai - Nature Protocols, 2022 - nature.com
With the development of a wide variety of animal models in recent years, there is a rapidly
growing demand for long-term, high-speed intravital fluorescence imaging to observe …

Development towards high-resolution kHz-speed rotation-free volumetric imaging

EM Asimakopoulou, V Bellucci, S Birnsteinova… - Optics …, 2024 - opg.optica.org
X-ray multi-projection imaging (XMPI) has the potential to provide rotation-free 3D movies of
optically opaque samples. The absence of rotation enables superior imaging speed and …

Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy

G de Vito, L Turrini, C Müllenbroich, P Ricci… - Biomedical optics …, 2022 - opg.optica.org
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional
imaging in zebrafish larvae. Conventional one-photon LSFM can however induce …