Advanced strategies in high-throughput droplet screening for enzyme engineering

W Yan, X Li, D Zhao, M Xie, T Li, L Qian, C Ye… - Biosensors and …, 2023 - Elsevier
Enzymes, as biocatalysts, play a cumulatively important role in environmental purification
and industrial production of chemicals and pharmaceuticals. However, natural enzymes are …

Surface patterning techniques for proteins on nano-and micro-systems: a modulated aspect in hierarchical structures

M Bhatt, P Shende - Journal of Materials Chemistry B, 2022 - pubs.rsc.org
The surface patterning of protein using fabrication or the external functionalization of
structures demonstrates various applications in the biomedical field for bioengineering …

Protein Dielectrophoresis: A Tale of Two Clausius-Mossottis—Or Something Else?

R Pethig - Micromachines, 2022 - mdpi.com
Standard DEP theory, based on the Clausius–Mossotti (CM) factor derived from solving the
boundary-value problem of macroscopic electrostatics, fails to describe the …

Trends and challenges in microfluidic methods for protein manipulation—A review

JM de los Santos‐Ramirez, PG Boyas‐Chavez… - …, 2024 - Wiley Online Library
Proteins are important molecules involved in an immensely large number of biological
processes. Being capable of manipulating proteins is critical for developing reliable and …

Nonlinear Electrokinetic Methods of Particles and Cells

BH Lapizco-Encinas - Annual Review of Analytical Chemistry, 2024 - annualreviews.org
Nonlinear electrokinetic phenomena offer label-free, portable, and robust approaches for
particle and cell assessment, including selective enrichment, separation, sorting, and …

Fluid-screen as a real time dielectrophoretic method for universal microbial capture

RE Weber, JJ Petkowski, B Michaels, K Wisniewski… - Scientific Reports, 2021 - nature.com
Bacterial culture methods, eg Plate Counting Method (PCM), are a gold standard in the
assessment of microbial contamination in multitude of human industries. They are however …

Protein albumin manipulation and electrical quantification of molecular dielectrophoresis responses for biomedical applications

NSA Nasir, R Deivasigamani, MFMR Wee, AA Hamzah… - Micromachines, 2022 - mdpi.com
Research relating to dielectrophoresis (DEP) has been progressing rapidly through time as
it is a strong and controllable technique for manipulation, separation, preconcentration, and …

Dielectrophoretic Movement of Cell Passing Between Surface Electrodes in Flow Channel

S Hashimoto, R Ono - Journal of Engineering and …, 2024 - asmedigitalcollection.asme.org
In this study, cell behavior in a microchannel was tracked for the application of
dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was …

Dielectrophoretic separation and purification: from colloid and biological particles to droplets

S Hu, Y Wang, Y Wang, X Chen, R Tong - Journal of Chromatography A, 2024 - Elsevier
It is indispensable to realize the high level of purification and separation, so that objective
particles, such as malignant cells, harmful bacteria, and special proteins or biological …

Direct in-situ capture, separation and visualization of biological particles with fluid-screen in the context of venus life finder mission concept study

RE Weber, JJ Petkowski, MU Weber - Aerospace, 2022 - mdpi.com
Evidence of chemical disequilibria and other anomalous observations in the Venusian
atmosphere motivate the search for life within the planet's temperate clouds. To find signs of …