Applications of western blot technique: From bench to bedside

GH Meftahi, Z Bahari… - Biochemistry and …, 2021 - Wiley Online Library
Western blot (WB) or immunoblot is a workhorse method. It is commonly used by biologists
for study of different aspects of protein biomolecules. In addition, it has been widely used in …

Progress on phage display technology: tailoring antibodies for cancer immunotherapy

RKA França, IC Studart, MRL Bezerra, LQ Pontes… - Viruses, 2023 - mdpi.com
The search for innovative anti-cancer drugs remains a challenge. Over the past three
decades, antibodies have emerged as an essential asset in successful cancer therapy. The …

Applications of the phage display technology in molecular biology, biotechnology and medicine

K Pierzynowska, J Morcinek-Orłowska… - Critical Reviews in …, 2023 - Taylor & Francis
The phage display technology is based on the presentation of peptide sequences on the
surface of virions of bacteriophages. Its development led to creation of sophisticated systems …

Human antibodies neutralizing diphtheria toxin in vitro and in vivo

EV Wenzel, M Bosnak, R Tierney, M Schubert… - Scientific reports, 2020 - nature.com
Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. The bacterium
primarily infects the throat and upper airways and the produced diphtheria toxin (DT), which …

Phage-displayed peptides for targeting tyrosine kinase membrane receptors in cancer therapy

A Aloisio, N Nisticò, S Mimmi, D Maisano, E Vecchio… - Viruses, 2021 - mdpi.com
Receptor tyrosine kinases (RTKs) regulate critical physiological processes, such as cell
growth, survival, motility, and metabolism. Abnormal activation of RTKs and relative …

A specific anti-cyclin D1 intrabody represses breast cancer cell proliferation by interrupting the cyclin D1–CDK4 interaction

J Zhao, Y Wu, T Xiao, C Cheng, T Zhang, Z Gao… - Breast Cancer Research …, 2023 - Springer
Background Cyclin D1 overexpression may contribute to development of various cancers,
including breast cancer, and thus may serve as a key cancer diagnostic marker and …

Pyruvate dehydrogenase complex—enzyme 2, a new target for Listeria spp. detection identified using combined phage display technologies

GMSG Moreira, SMS Köllner, S Helmsing, L Jänsch… - Scientific Reports, 2020 - nature.com
The genus Listeria comprises ubiquitous bacteria, commonly present in foods and food
production facilities. In this study, three different phage display technologies were employed …

Understanding and modulating antibody fine specificity: lessons from combinatorial biology

G Rojas - Antibodies, 2022 - mdpi.com
Combinatorial biology methods such as phage and yeast display, suitable for the generation
and screening of huge numbers of protein fragments and mutated variants, have been …

Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

S Zhao, G Yang, X Xie, G Yan, F Wang, W Chen, L Ma - Biomolecules, 2022 - mdpi.com
Adenosine triphosphate (ATP), as a universal energy currency, takes a central role in many
biochemical reactions with potential for the synthesis of numerous high-value products …

Identification of Heterophilic Epitopes of H1N1 Influenza Virus Hemagglutinin

J Sun, C Guo, G Wang, L Yan, Q Feng, Y Li, X Huo… - Current …, 2023 - Springer
Our previous studies found that the H1-50 monoclonal antibody (mAb) of influenza A virus
hemagglutinin (HA) cross-reacted with pancreatic tissue and islet β-cells, and further studies …