The genomic integrity of Mycobacterium tuberculosis is continuously threatened by the
harsh survival conditions inside host macrophages, due to immune and antibiotic stresses …

The well-being of all living organisms relies on the accurate duplication of their genomes.
This is usually achieved by highly elaborate replicase complexes which ensure that this task …

Ribonuclease HII (RNaseHII) is the principal enzyme that removes misincorporated
ribonucleoside monophosphates (rNMPs) from genomic DNA. Here, we present structural …

Ribonucleotides frequently contaminate DNA and, if not removed, cause genomic instability.
Consequently, all organisms are equipped with RNase H enzymes to remove RNA-DNA …

The current model for Okazaki fragment maturation in bacteria invokes RNA cleavage by
RNase H, followed by strand displacement synthesis and 5′ RNA flap removal by DNA …

Ultraviolet (UV) induces pyrimidine dimers (PDs) in DNA and replication-dependent
fragmentation in chromosomes. The rnhAB mutants in Escherichia coli, accumulating R …

The rnhAB mutant Escherichia coli, deficient in two RNase H enzymes that remove both R-
loops and incorporated ribonucleotides (rNs) from DNA, grow slowly, suggesting …

Modified nucleoside triphosphates (NTPs) are invaluable tools to probe bacterial enzymatic
mechanisms, develop novel genetic material, and engineer drugs and proteins with new …

Research on Escherichia coli DNA replication paved the groundwork for many breakthrough
discoveries with important implications for our understanding of human molecular biology …

Bacillus subtilis PcrA interacts with the RNA polymerase and might contribute to mitigate
replication–transcription conflicts (RTCs). We show that PcrA depletion lethality is partially …