Single-cell sorting based on secreted products for functionally defined cell therapies
Cell therapies have emerged as a promising new class of “living” therapeutics over the last
decade and have been particularly successful for treating hematological malignancies …
decade and have been particularly successful for treating hematological malignancies …
Flow lithography for structured microparticles: fundamentals, methods and applications
Structured microparticles, with unique shapes, customizable sizes, multiple materials, and
spatially-defined chemistries, are leading the way for emerging 'lab on a …
spatially-defined chemistries, are leading the way for emerging 'lab on a …
Associating growth factor secretions and transcriptomes of single cells in nanovials using SEC-seq
Cells secrete numerous bioactive molecules that are essential for the function of healthy
organisms. However, scalable methods are needed to link individual cell secretions to their …
organisms. However, scalable methods are needed to link individual cell secretions to their …
Suspendable hydrogel nanovials for massively parallel single-cell functional analysis and sorting
Techniques to analyze and sort single cells based on functional outputs, such as secreted
products, have the potential to transform our understanding of cellular biology as well as …
products, have the potential to transform our understanding of cellular biology as well as …
SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells
RYH Cheng, J de Rutte, CEK Ito, AR Ott… - Nature …, 2023 - nature.com
The secreted products of cells drive many functions in vivo; however, methods to link this
functional information to surface markers and transcriptomes have been lacking. By …
functional information to surface markers and transcriptomes have been lacking. By …
Defining T cell receptor repertoires using nanovial-based binding and functional screening
The ability to selectively bind to antigenic peptides and secrete effector molecules can
define rare and low-affinity populations of cells with therapeutic potential in emerging T cell …
define rare and low-affinity populations of cells with therapeutic potential in emerging T cell …
A SERS biosensor based on aptamer-based Fe3O4@ SiO2@ Ag magnetic recognition and embedded SERS probes for ultrasensitive simultaneous detection of …
F Mi, M Guan, Y Wang, G Chen, PF Geng - Microchemical Journal, 2023 - Elsevier
A rapid and sensitive SERS biosensor based on aptamer-modified Fe 3 O 4@ SiO 2@ Ag
and Au-MPBA/DTNB@ Ag was constructed for simultaneous detection of Staphylococcus …
and Au-MPBA/DTNB@ Ag was constructed for simultaneous detection of Staphylococcus …
Lab on a particle technologies
From “Lab on a Chip” to “Lab on a Particle”. The miniaturization and automation of life
science laboratory operations has leveraged fabrication advances in the integrated circuit …
science laboratory operations has leveraged fabrication advances in the integrated circuit …
Integration of three non-interfering SERS probes combined with ConA-functionalized magnetic nanoparticles for extraction and detection of multiple foodborne …
F Mi, M Guan, Y Wang, G Chen, P Geng, C Hu - Microchimica Acta, 2023 - Springer
A sandwich-structured SERS biosensor has been constructed for simultaneous detection of
multiple pathogenic bacteria, consisting of non-interfering SERS probes for bacterial …
multiple pathogenic bacteria, consisting of non-interfering SERS probes for bacterial …
Optimizing cell therapy by sorting cells with high extracellular vesicle secretion
Critical challenges remain in clinical translation of extracellular vesicle (EV)-based
therapeutics due to the absence of methods to enrich cells with high EV secretion. Current …
therapeutics due to the absence of methods to enrich cells with high EV secretion. Current …