Bioconjugation methodologies have proven to play a central enabling role in the recent
development of biotherapeutics and chemical biology approaches. Recent endeavours in …

Infrared difference spectroscopy probes vibrational changes of proteins upon their
perturbation. Compared with other spectroscopic methods, it stands out by its sensitivity to …

INTRODUCTION Photoenzymes are rare biocatalysts driven by absorption of a photon at
each catalytic cycle; they inspire development of artificial photoenzymes with valuable …

Using complementary approaches of potentiometry and NMR spectroscopy, we have
determined that the equilibrium acid dissociation constant (pKa value) of the arginine …

Life on earth is almost entirely solar-powered. We can get some idea of the enormous
quantity of energy received from the sun by noting that during daylight hours, the sun …

The p K a values and charge states of ionizable residues in polypeptides and proteins are
frequently determined via NMR-monitored pH titrations. To aid the interpretation of the …

Rhodopsins had long been considered non-fluorescent until a peculiar voltage-sensitive
fluorescence was reported for archaerhodopsin-3 (Arch3) derivatives. These proteins …

The steps in the mechanism of proton transport in bacteriorhodopsin include examples for
most kinds of proton transfer reactions that might occur in a transmembrane pump: proton …

Proton transfer is crucial for many enzyme reactions. Here, we show that in addition to
protonatable amino acid side chains, water networks could constitute proton-binding sites in …

DypB from Rhodococcus jostii RHA1 is a bacterial dye-decolorizing peroxidase (DyP) that
oxidizes lignin and Mn (II). Three residues interact with the iron-bound solvent species in …