Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues
The production of biofuels as an efficient source of renewable energy has received
considerable attention due to increasing energy demands and regulatory incentives to …
considerable attention due to increasing energy demands and regulatory incentives to …
Development of a spore-based mucosal vaccine against the bovine respiratory pathogen Mannheimia haemolytica
Bovine respiratory disease (BRD) is a significant health issue in the North American feedlot
industry, causing substantial financial losses due to morbidity and mortality. A lack of …
industry, causing substantial financial losses due to morbidity and mortality. A lack of …
Application of transcriptomics to compare the carbohydrate active enzymes that are expressed by diverse genera of anaerobic fungi to degrade plant cell wall …
RJ Gruninger, TTM Nguyen, ID Reid, JL Yanke… - Frontiers in …, 2018 - frontiersin.org
The efficiency with which the anaerobic fungi (phylum Neocallimastigomycota) degrade
plant biomass is well-recognized and in recent years has received renewed interest. To …
plant biomass is well-recognized and in recent years has received renewed interest. To …
SACCHARIS: an automated pipeline to streamline discovery of carbohydrate active enzyme activities within polyspecific families and de novo sequence datasets
DR Jones, D Thomas, N Alger, A Ghavidel… - Biotechnology for …, 2018 - Springer
Background Deposition of new genetic sequences in online databases is expanding at an
unprecedented rate. As a result, sequence identification continues to outpace functional …
unprecedented rate. As a result, sequence identification continues to outpace functional …
Bifidobacterial GH146 β-l-arabinofuranosidase for the removal of β1,3-l-arabinofuranosides on plant glycans
K Fujita, H Tsunomachi, P Lixia, S Maruyama… - Applied Microbiology …, 2024 - Springer
Abstract l-Arabinofuranosides with β-linkages are present in several plant molecules, such
as arabinogalactan proteins (AGPs), extensin, arabinan, and rhamnogalacturonan-II. We …
as arabinogalactan proteins (AGPs), extensin, arabinan, and rhamnogalacturonan-II. We …
Characterization of the dynamic changes of ruminal microbiota colonizing citrus pomace waste during rumen incubation for volatile fatty acid production
S Yu, L Li, H Zhao, Y Tu, M Liu, L Jiang… - Microbiology …, 2023 - Am Soc Microbiol
Rumen microorganisms are promising for efficient bioconversion of lignocellulosic wastes to
biofuels and industrially relevant products. Investigating the dynamic changes of the rumen …
biofuels and industrially relevant products. Investigating the dynamic changes of the rumen …
Biochemical unravelling of the endoxylanase activity in a bifunctional GH39 enzyme cloned and expressed from thermophilic Geobacillus sp. WSUCF1
The glycoside hydrolase family 39 (GH39) proteins are renowned for their extremophilic and
multifunctional enzymatic properties, yet the molecular mechanisms underpinning these …
multifunctional enzymatic properties, yet the molecular mechanisms underpinning these …
Open prediction of polysaccharide utilisation loci (PUL) in 5414 public Bacteroidetes genomes using PULpy
Polysaccharide utilisation loci (PUL) are regions within bacterial genomes that encode all
the necessary machinery for the cleavage of particular carbohydrates. For the Bacteroidetes …
the necessary machinery for the cleavage of particular carbohydrates. For the Bacteroidetes …
Novel 3-O-α-d-Galactosyl-α-l-Arabinofuranosidase for the Assimilation of Gum Arabic Arabinogalactan Protein in Bifidobacterium longum subsp. longum
Y Sasaki, A Horigome, T Odamaki, JZ Xiao… - Applied and …, 2021 - Am Soc Microbiol
Gum arabic arabinogalactan (AG) protein (AGP) is a unique dietary fiber that is degraded
and assimilated by only specific strains of Bifidobacterium longum subsp. longum. Here, we …
and assimilated by only specific strains of Bifidobacterium longum subsp. longum. Here, we …
Importance of accessory enzymes in hemicellulose degradation
V Puchart, K Šuchová, P Biely - Polysaccharide-Degrading Biocatalysts, 2023 - Elsevier
This chapter reviews microbial enzymes degrading two major plant hemicelluloses, xylan
and β-mannan. The main chains of these two polysaccharides are built of β-1, 4 …
and β-mannan. The main chains of these two polysaccharides are built of β-1, 4 …