Structured illumination microscopy (SIM) has become the standard for next-generation wide-
field microscopy, offering ultrahigh imaging speed, superresolution, a large field-of-view …

Remarkable progress in ageing research has been achieved over the past decades.
General perceptions and experimental evidence pinpoint that the decline of physical …

A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is
the maximum photon flux that can be collected. To further increase the effective resolution …

The goal when imaging bioprocesses with optical microscopy is to acquire the most
spatiotemporal information with the least invasiveness. Deep neural networks have …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

Deep neural networks have enabled astonishing transformations from low-resolution (LR) to
super-resolved images. However, whether, and under what imaging conditions, such deep …

A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …

Organelles compartmentalize eukaryotic cells, enhancing their ability to respond to
environmental and developmental changes. One way in which organelles communicate and …

The mechanism that initiates autophagosome formation on the ER in multicellular organisms
is elusive. Here, we showed that autophagy stimuli trigger Ca 2+ transients on the outer …

Structured illumination microscopy (SIM) is one of the powerful super-resolution modalities
in bioscience with the advantages of full-field imaging and high photon efficiency. However …