A user's guide to golden gate cloning methods and standards

JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

Plant synthetic biology innovations for biofuels and bioproducts

Y Yang, TA Chaffin, AH Ahkami, E Blumwald… - Trends in …, 2022 - cell.com
Plant-based biosynthesis of fuels, chemicals, and materials promotes environmental
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …

Optogenetic control of gene expression in plants in the presence of ambient white light

R Ochoa-Fernandez, NB Abel, FG Wieland… - Nature …, 2020 - nature.com
Optogenetics is the genetic approach for controlling cellular processes with light. It provides
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …

COP1 destabilizes DELLA proteins in Arabidopsis

N Blanco-Touriñán, M Legris… - Proceedings of the …, 2020 - National Acad Sciences
DELLA transcriptional regulators are central components in the control of plant growth
responses to the environment. This control is considered to be mediated by changes in the …

Rational design of minimal synthetic promoters for plants

YM Cai, K Kallam, H Tidd, G Gendarini… - Nucleic Acids …, 2020 - academic.oup.com
Promoters serve a critical role in establishing baseline transcriptional capacity through the
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …

Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoor farms into biomanufacturing facilities

B Diego-Martin, B González, M Vazquez-Vilar… - Frontiers in plant …, 2020 - frontiersin.org
The current CoVid-19 crisis is revealing the strengths and the weaknesses of the world's
capacity to respond to a global health crisis. A critical weakness has resulted from the …

Making small molecules in plants: a chassis for synthetic biology‐based production of plant natural products

X Liu, P Zhang, Q Zhao… - Journal of Integrative Plant …, 2023 - Wiley Online Library
Plant natural products have been extensively exploited in food, medicine, flavor, cosmetic,
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …

Assessment of Cas12a‐mediated gene editing efficiency in plants

JM Bernabé‐Orts, I Casas‐Rodrigo… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering.
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …

Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery

A Jozwiak, PD Sonawane, S Panda… - Nature Chemical …, 2020 - nature.com
Glycosylation is one of the most prevalent molecular modifications in nature. Single or
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …

Engineering the Biosynthesis of Late-Stage Vinblastine Precursors Precondylocarpine Acetate, Catharanthine, Tabersonine in Nicotiana benthamiana

D Grzech, B Hong, L Caputi, PD Sonawane… - ACS Synthetic …, 2022 - ACS Publications
Vinblastine is a chemotherapy agent produced by the plant Catharanthus roseus in small
quantities. Currently, vinblastine is sourced by isolation or semisynthesis. Nicotiana …