A user's guide to golden gate cloning methods and standards
JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …
of applications has driven the development of many different cloning strategies. One method …
Plant synthetic biology innovations for biofuels and bioproducts
Plant-based biosynthesis of fuels, chemicals, and materials promotes environmental
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
sustainability, which includes decreases in greenhouse gas emissions, water pollution, and …
Optogenetic control of gene expression in plants in the presence of ambient white light
Optogenetics is the genetic approach for controlling cellular processes with light. It provides
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …
COP1 destabilizes DELLA proteins in Arabidopsis
N Blanco-Touriñán, M Legris… - Proceedings of the …, 2020 - National Acad Sciences
DELLA transcriptional regulators are central components in the control of plant growth
responses to the environment. This control is considered to be mediated by changes in the …
responses to the environment. This control is considered to be mediated by changes in the …
Rational design of minimal synthetic promoters for plants
YM Cai, K Kallam, H Tidd, G Gendarini… - Nucleic Acids …, 2020 - academic.oup.com
Promoters serve a critical role in establishing baseline transcriptional capacity through the
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …
recruitment of proteins, including transcription factors. Previously, a paucity of data for cis …
Pilot production of SARS-CoV-2 related proteins in plants: a proof of concept for rapid repurposing of indoor farms into biomanufacturing facilities
B Diego-Martin, B González, M Vazquez-Vilar… - Frontiers in plant …, 2020 - frontiersin.org
The current CoVid-19 crisis is revealing the strengths and the weaknesses of the world's
capacity to respond to a global health crisis. A critical weakness has resulted from the …
capacity to respond to a global health crisis. A critical weakness has resulted from the …
Making small molecules in plants: a chassis for synthetic biology‐based production of plant natural products
X Liu, P Zhang, Q Zhao… - Journal of Integrative Plant …, 2023 - Wiley Online Library
Plant natural products have been extensively exploited in food, medicine, flavor, cosmetic,
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …
renewable fuel, and other industrial sectors. Synthetic biology has recently emerged as a …
Assessment of Cas12a‐mediated gene editing efficiency in plants
JM Bernabé‐Orts, I Casas‐Rodrigo… - Plant biotechnology …, 2019 - Wiley Online Library
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering.
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …
To obtain a comparative assessment of RNA‐guided endonuclease (RGEN) types in plants …
Plant terpenoid metabolism co-opts a component of the cell wall biosynthesis machinery
Glycosylation is one of the most prevalent molecular modifications in nature. Single or
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …
multiple sugars can decorate a wide range of acceptors from proteins to lipids, cell wall …
Engineering the Biosynthesis of Late-Stage Vinblastine Precursors Precondylocarpine Acetate, Catharanthine, Tabersonine in Nicotiana benthamiana
Vinblastine is a chemotherapy agent produced by the plant Catharanthus roseus in small
quantities. Currently, vinblastine is sourced by isolation or semisynthesis. Nicotiana …
quantities. Currently, vinblastine is sourced by isolation or semisynthesis. Nicotiana …