Human pluripotent stem cell culture: current status, challenges, and advancement

S Dakhore, B Nayer, K Hasegawa - Stem cells international, 2018 - Wiley Online Library
Over the past two decades, human embryonic stem cells (hESCs) have gained attention due
to their pluripotent and proliferative ability which enables production of almost all cell types …

Human embryonic stem cells: derivation, culture, and differentiation: a review

T Vazin, WJ Freed - Restorative neurology and neuroscience, 2010 - content.iospress.com
The greatest therapeutic promise of human embryonic stem cells (hESC) is to generate
specialized cells to replace damaged tissue in patients suffering from various degenerative …

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

T Hyvärinen, A Hyysalo, FE Kapucu, L Aarnos… - Scientific reports, 2019 - nature.com
Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in
vitro modeling of neurological diseases and for advancing drug development and …

Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder‐free; medium with fetal calf …

F Mannello, GA Tonti - Stem cells, 2007 - academic.oup.com
The choice of an optimal strategy of stem cell culture is at the moment an impossible task,
and the elaboration of a culture medium adapted to the production of embryonic and adult …

Good manufacturing practice and clinical-grade human embryonic stem cell lines

C Unger, H Skottman, P Blomberg… - Human molecular …, 2008 - academic.oup.com
Human embryonic stem cell (hESC) lines, after directed differentiation, hold the greatest
potential for cell transplantation treatment in many severe diseases. Good manufacturing …

The generation of six clinical-grade human embryonic stem cell lines

JM Crook, TT Peura, L Kravets, AG Bosman… - Cell stem cell, 2007 - cell.com
The edict for producing clinically compliant human embryonic stem cells (hESCs)
necessitates adherence to global ethical standards for egg procurement and embryo …

Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems

N Desai, P Rambhia, A Gishto - Reproductive Biology and Endocrinology, 2015 - Springer
Human embryonic stem cells (hESC) have emerged as attractive candidates for cell-based
therapies that are capable of restoring lost cell and tissue function. These unique cells are …

A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cells

K Rajala, B Lindroos, SM Hussein, RS Lappalainen… - PloS one, 2010 - journals.plos.org
Background The growth of stem cells in in vitro conditions requires optimal balance between
signals mediating cell survival, proliferation, and self-renewal. For clinical application of …

Derivation of xeno-free and GMP-grade human embryonic stem cells–platforms for future clinical applications

SE Tannenbaum, T Tako Turetsky, O Singer… - PloS one, 2012 - journals.plos.org
Clinically compliant human embryonic stem cells (hESCs) should be developed in
adherence to ethical standards, without risk of contamination by adventitious agents. Here …

Generation of human‐induced pluripotent stem cells by a nonintegrating RNA Sendai virus vector in feeder‐free or xeno‐free conditions

CC MacArthur, A Fontes, N Ravinder… - Stem cells …, 2012 - Wiley Online Library
The generation of induced pluripotent stem cells (iPSCs) from somatic cells has enabled the
possibility of providing unprecedented access to patient‐specific iPSC cells for drug …