CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-
associated nuclease) defense systems have been naturally coopted for guide RNA–directed …

Most life forms harbor multiple, diverse mobile genetic elements (MGE) that widely differ in
their rates and mechanisms of mobility. Recent findings on two classes of MGE in …

CRISPR-associated transposases (CASTs) repurpose nuclease-deficient CRISPR effectors
to catalyze RNA-guided transposition of large genetic payloads. Type VK CASTs offer …

Synthetic perturbation of gene expression is central to our ability to reliably uncover
genotype–phenotype relationships in microbes. Here, we present a novel transcription …

The Tn7 family of transposons is notable for its highly regulated integration mechanisms,
including programmable RNA-guided transposition. The targeting pathways rely on …

Mobile genetic elements play an important role in the acquisition of antibiotic and biocide
resistance, especially through the formation of resistance islands in bacterial chromosomes …

CRISPR-Cas systems revolutionized the genome engineering field but need to induce
double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size …

The clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated
(Cas) system is a powerful genomic DNA editing tool. The increased applications of gene …