Recent experimental developments in genome-wide RNA quantification hold considerable
promise for systems biology. However, rigorously probing the biology of living cells requires …

Assays such as CITE-seq can measure the abundance of cell surface proteins on individual
cells using antibody derived tags (ADTs). However, many ADTs have high levels of …

Here, we examine how prenatal inflammation shapes tissue function and immunity in the
lung by reprogramming tissue-resident immune cells from early development. Maternal, but …

Single-cell RNA-sequencing (scRNA-seq) presents an opportunity to deconstruct cellular
networks but is limited by the loss of biological information, including in vivo cellular states …

Alveologenesis, the final stage in lung development, substantially remodels the distal lung,
expanding the alveolar surface area for efficient gas exchange. Secondary crest …

In droplet-based single-cell and single-nucleus RNA-seq assays, systematic contamination
of ambient RNA molecules biases the quantification of gene expression levels. Existing …

Following acute retinal damage, zebrafish possess the ability to regenerate all neuronal
subtypes through Müller glia (MG) reprogramming and asymmetric cell division that …

Following acute retinal damage, zebrafish possess the ability to regenerate all neuronal
subtypes. This regeneration requires Müller glia (MG) to reprogram and divide …

Autoreactive B cells play an important but ill-defined role in autoimmune type 1 diabetes
(T1D). To better understand their contribution, we performed single cell gene and BCR-seq …

Single-cell transcriptomics has emerged as the preferred tool to define cell identity through
the analysis of gene expression signatures. However, there are limited studies that have …