Recent advances in mass spectrometry analysis of neuropeptides

A Phetsanthad, NQ Vu, Q Yu… - Mass spectrometry …, 2023 - Wiley Online Library
Due to their involvement in numerous biochemical pathways, neuropeptides have been the
focus of many recent research studies. Unfortunately, classic analytical methods, such as …

Current perspectives on supercharging reagents in electrospray ionization mass spectrometry

DA Abaye, IA Agbo, BV Nielsen - RSC advances, 2021 - pubs.rsc.org
In electrospray ionization mass spectrometry (ESI-MS), analytes are introduced into the
mass spectrometer in typically aqueous-organic solvent mixtures, including pH modifiers …

Supercharging with m-Nitrobenzyl Alcohol and Propylene Carbonate: Forming Highly Charged Ions with Extended, Near-Linear Conformations

CC Going, ER Williams - Analytical chemistry, 2015 - ACS Publications
The effectiveness of the supercharging reagents m-nitrobenzyl alcohol (m-NBA) and
propylene carbonate at producing highly charged protein ions in electrospray ionization is …

Parasympathetic neurons in the human submandibular ganglion

M Kawashima, T Yajima, D Tachiya, S Kokubun… - Tissue and Cell, 2021 - Elsevier
The submandibular ganglion (SMG) contains parasympathetic neurons which innervate the
submandibular gland. In this study, immunohistochemistry for vasoactive intestinal …

BSA fragmentation specifically induced by added electrolytes: An electrospray ionization mass spectrometry investigation

G Lusci, T Pivetta, C Carucci, DF Parsons… - Colloids and Surfaces B …, 2022 - Elsevier
Biointerfaces are significantly affected by electrolytes according to the Hofmeister series.
This work reports a systematic investigation on the effect of different metal chlorides, sodium …

Weak acid-base interactions of histidine and cysteine affect the charge states, tertiary structure, and Zn (II)-binding of heptapeptides

YF Lin, EN Yousef, E Torres, L Truong… - Journal of The …, 2019 - ACS Publications
Zinc fingers are proteins that are characterized by the coordination of zinc ions by an amino
acid sequence that commonly contains two histidines and two cysteines (2His-2Cys motif) …

Supercharging and multiple reaction monitoring of high‐molecular‐weight intact proteins using triple quadrupole mass spectrometry

DD Khanal, YZ Baghdady, BJ Figard… - … in Mass Spectrometry, 2019 - Wiley Online Library
Rationale Different supercharging agents were tested to study their effect on the intensity
and charge state distributions of high‐molecular‐weight intact proteins. The goal of this work …

Exploring the dynamic landscape of immunopeptidomics: Unravelling posttranslational modifications and navigating bioinformatics terrain

D Flender, F Vilenne, C Adams… - Mass Spectrometry …, 2024 - Wiley Online Library
Immunopeptidomics is becoming an increasingly important field of study. The capability to
identify immunopeptides with pivotal roles in the human immune system is essential to shift …

A comprehensive approach to detecting multitudinous bioactive peptides in equine plasma and urine using hydrophilic interaction liquid chromatography coupled to …

F Guan, Y You, X Li, MA Robinson - Drug testing and analysis, 2019 - Wiley Online Library
Bioactive peptides possess pharmacological effects and can be illicitly used in sports. To
deter such misuse, an untargeted method using high resolution mass spectrometry (HRMS) …

Challenges for The In Vivo Quantification of Brain Neuropeptides Using Microdialysis Sampling and LC–MS

Y Van Wanseele, A De Prins, D De Bundel… - Bioanalysis, 2016 - Taylor & Francis
In recent years, neuropeptides and their receptors have received an increased interest in
neuropharmacological research. Although these molecules are considered relatively small …