Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

M Bottini, S Mebarek, KL Anderson… - … et Biophysica Acta (BBA …, 2018 - Elsevier
Abstract Background Matrix vesicles (MVs) are released from hypertrophic chondrocytes
and from mature osteoblasts, the cells responsible for endochondral and membranous …

Biophysical aspects of biomineralization

M Bolean, AMS Simão, MB Barioni, BZ Favarin… - Biophysical …, 2017 - Springer
During the process of endochondral bone formation, chondrocytes and osteoblasts
mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) …

Proteoliposomes in nanobiotechnology

P Ciancaglini, AMS Simão, M Bolean, JL Millán… - Biophysical …, 2012 - Springer
Proteoliposomes are systems that mimic lipid membranes (liposomes) to which a protein
has been incorporated or inserted. During the last decade, these systems have gained …

Overview on solubilization and lipid reconstitution of Na, K-ATPase: enzyme kinetic and biophysical characterization

JS Yoneda, HG Sebinelli, R Itri, P Ciancaglini - Biophysical Reviews, 2020 - Springer
Na, K-ATPase is a membrane protein which plays a vital role. It pumps Na+ and K+ ions
across the cellular membranes using energy from ATP hydrolysis, and is responsible for …

Topographical and mechanical properties of liposome surfaces harboring Na, K-ATPase by means of atomic force microscopy

HG Sebinelli, IA Borin, P Ciancaglini, M Bolean - Soft Matter, 2019 - pubs.rsc.org
In this study, we obtained unprecedented AFM images of the Na, K-ATPase (NKA) pump
after being reconstituted into DPPC and DPPC: DPPE liposomes. The mechanical …

[HTML][HTML] Na, K-ATPase reconstituted in ternary liposome: The presence of cholesterol affects protein activity and thermal stability

JS Yoneda, CF Rigos, TFA de Lourenço… - Archives of biochemistry …, 2014 - Elsevier
Differential scanning calorimetry (DSC) was applied to investigate the effect of cholesterol
on the thermotropic properties of the lipid membrane (DPPC and DPPE). The thermostability …

Multimeric species in equilibrium in detergent-solubilized Na, K-ATPase

JS Yoneda, G Scanavachi, HG Sebinelli… - International journal of …, 2016 - Elsevier
In this work, we find an equilibrium between different Na, K-ATPase (NKA) oligomeric
species solubilized in a non-ionic detergent C 12 E 8 by means of Dynamic Light Scattering …

[HTML][HTML] Addition of subunit γ, K+ ions, and lipid restores the thermal stability of solubilized Na, K-ATPase

JS Yoneda, CF Rigos, P Ciancaglini - Archives of biochemistry and …, 2013 - Elsevier
Differential scanning calorimetry (DSC) was applied to ascertain the effect caused by K+,
Na+, ATP, detergent, DPPC, DPPE, and subunit γ on the thermostability of Na, K-ATPase …

Unraveling the Na,K-ATPase α4 Subunit Assembling Induced by Large Amounts of C12E8 by Means of Small-Angle X-ray Scattering

LRS Barbosa, CF Rigos, JS Yoneda, R Itri… - The Journal of …, 2010 - ACS Publications
In the current work, we studied the effect of the nonionic detergent
dodecyloctaethyleneglycol, C12E8, on the structure and oligomeric form of the Na, K …

Estudos biofísicos da interação entre a Na, K-ATPase ea subunidade gama, ATP, Na+, K+, DPPC, DPPE e colesterol

JS Yoneda - 2014 - teses.usp.br
A Na, K-ATPase (NKA) é expressa em todas as células animais e é um dos membros mais
importantes da família das ATPases do tipo P. Consiste de três subunidades: α, β e γ. Essa …