Three-photon microscopy has been increasingly adopted for probing neural activities
beyond the typical two-photon imaging depth. In this review, we outline the unique …

The development of three-photon microscopy (3PM) has greatly expanded the capability of
imaging deep within biological tissues, enabling neuroscientists to visualize the structure …

3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and
highly scattering samples. To date, 3-photon excitation has been restricted to scanning a …

We developed a multiphoton imaging method to capture neural structure and activity in
behaving flies through the intact cuticle. Our measurements showed that the fly head cuticle …

The intrinsic fluorescence of bacterial samples has a proven potential for label-free bacterial
characterization, monitoring bacterial metabolic functions, and as a mechanism for tracking …

A limitation of current methods for examining neural activity over long periods of time in the
fly, Drosophila melanogaster, is the need to remove the head cuticle and the underlying …