The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Forward genetic screens are a powerful and unbiased approach for uncovering the genetic
basis behind a specific phenotype. Genome-wide mutagenesis followed by phenotypic …

Multiple sclerosis (MS) involves the infiltration of autoreactive T cells into the CNS, yet we
lack a comprehensive understanding of the signaling pathways that regulate this process …

Background Chemogenetic profiling enables the identification of gene mutations that
enhance or suppress the activity of chemical compounds. This knowledge provides insights …

Genome-wide mutational screens are central to understanding the genetic underpinnings of
evolved and engineered phenotypes. The widespread adoption of CRISPR-Cas9 genome …

Combinatorial CRISPR-Cas screens have advanced the mapping of genetic interactions,
but their experimental scale limits the number of targetable gene combinations. Here, we …

The genetic basis of naive pluripotency maintenance and loss is a central question in
embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse …

Background Genome editing by CRISPR-Cas9 technology allows large-scale screening of
gene essentiality in cancer. A confounding factor when interpreting CRISPR-Cas9 screens …

Genome-wide CRISPR/Cas9 knockout screens are revolutionizing mammalian functional
genomics. However, their range of applications remains limited by signal variability from …

Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed,
high-throughput screening strategy that minimizes library size without sacrificing gene …