[HTML][HTML] Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
[HTML][HTML] A genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model
A Kendirli, C de la Rosa, KF Lämmle, K Eglseer… - Nature …, 2023 - nature.com
Multiple sclerosis (MS) involves the infiltration of autoreactive T cells into the CNS, yet we
lack a comprehensive understanding of the signaling pathways that regulate this process …
lack a comprehensive understanding of the signaling pathways that regulate this process …
[HTML][HTML] Identifying chemogenetic interactions from CRISPR screens with drugZ
Background Chemogenetic profiling enables the identification of gene mutations that
enhance or suppress the activity of chemical compounds. This knowledge provides insights …
enhance or suppress the activity of chemical compounds. This knowledge provides insights …
Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica
C Schwartz, JF Cheng, R Evans, CA Schwartz… - Metabolic …, 2019 - Elsevier
Genome-wide mutational screens are central to understanding the genetic underpinnings of
evolved and engineered phenotypes. The widespread adoption of CRISPR-Cas9 genome …
evolved and engineered phenotypes. The widespread adoption of CRISPR-Cas9 genome …
Minimized combinatorial CRISPR screens identify genetic interactions in autophagy
V Diehl, M Wegner, P Grumati, K Husnjak… - Nucleic acids …, 2021 - academic.oup.com
Combinatorial CRISPR-Cas screens have advanced the mapping of genetic interactions,
but their experimental scale limits the number of targetable gene combinations. Here, we …
but their experimental scale limits the number of targetable gene combinations. Here, we …
[HTML][HTML] Genome-wide CRISPR-KO screen uncovers mTORC1-mediated Gsk3 regulation in naive pluripotency maintenance and dissolution
The genetic basis of naive pluripotency maintenance and loss is a central question in
embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse …
embryonic stem cell biology. Here, we deploy CRISPR-knockout-based screens in mouse …
[HTML][HTML] Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting
Background Genome editing by CRISPR-Cas9 technology allows large-scale screening of
gene essentiality in cancer. A confounding factor when interpreting CRISPR-Cas9 screens …
gene essentiality in cancer. A confounding factor when interpreting CRISPR-Cas9 screens …
JACKS: joint analysis of CRISPR/Cas9 knockout screens
Genome-wide CRISPR/Cas9 knockout screens are revolutionizing mammalian functional
genomics. However, their range of applications remains limited by signal variability from …
genomics. However, their range of applications remains limited by signal variability from …
[HTML][HTML] Pooled library screening with multiplexed Cpf1 library
Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed,
high-throughput screening strategy that minimizes library size without sacrificing gene …
high-throughput screening strategy that minimizes library size without sacrificing gene …