[HTML][HTML] Engineering of thermostable enzymes for industrial applications
The catalytic properties of some selected enzymes have long been exploited to carry out
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
L Li, X Liu, Y Bai, B Yao, H Luo, T Tu - Journal of Agricultural and …, 2024 - ACS Publications
The acquisition of a thermostable enzyme is an indispensable prerequisite for its successful
implementation in industrial applications and the development of novel functionalities …
implementation in industrial applications and the development of novel functionalities …
Efficient production of protocatechuic acid using systems engineering of Escherichia coli
M Wang, H Wang, C Gao, W Wei, J Liu, X Chen… - Metabolic …, 2024 - Elsevier
Abstract Protocatechuic acid (3, 4-dihydroxybenzoic acid, PCA) is widely used in the
pharmaceuticals, health food, and cosmetics industries owing to its diverse biological …
pharmaceuticals, health food, and cosmetics industries owing to its diverse biological …
[HTML][HTML] Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
L FM Machado, A Currin, N Dixon - Journal of biological engineering, 2019 - Springer
Background Transcription factor-based biosensors are useful tools for the detection of
metabolites and industrially valuable molecules, and present many potential applications in …
metabolites and industrially valuable molecules, and present many potential applications in …
Rational engineering of p‐hydroxybenzoate hydroxylase to enable efficient gallic acid synthesis via a novel artificial biosynthetic pathway
Gallic acid (GA) is a naturally occurring phytochemical that has strong antioxidant and
antibacterial activities. It is also used as a potential platform chemical for the synthesis of …
antibacterial activities. It is also used as a potential platform chemical for the synthesis of …
[HTML][HTML] Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations
HM Huttanus, EKH Triola… - … in Bioengineering and …, 2023 - frontiersin.org
Targeted mutagenesis of a promoter or gene is essential for attaining new functions in
microbial and protein engineering efforts. In the burgeoning field of synthetic biology …
microbial and protein engineering efforts. In the burgeoning field of synthetic biology …
[HTML][HTML] A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution
RK Jha, JM Bingen, CW Johnson, TL Kern… - Metabolic engineering …, 2018 - Elsevier
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput
strain improvement in synthetic biology. Many biosensors are developed in model …
strain improvement in synthetic biology. Many biosensors are developed in model …
Proteins from Thermophilic Thermus thermophilus Often Do Not Fold Correctly in a Mesophilic Expression System Such as Escherichia coli
Majority of protein structure studies use Escherichia coli (E. coli) and other model organisms
as expression systems for other species' genes. However, protein folding depends on …
as expression systems for other species' genes. However, protein folding depends on …
Sensor-enabled alleviation of product inhibition in chorismate pyruvate-lyase
Product inhibition is a frequent bottleneck in industrial enzymes, and testing mutations to
alleviate product inhibition via traditional methods remains challenging as many variants …
alleviate product inhibition via traditional methods remains challenging as many variants …
[HTML][HTML] Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid
EA Shmonova, OV Voloshina, MV Ovsienko… - PLoS …, 2020 - journals.plos.org
The dehydroshikimate dehydratase (DSD) from Corynebacterium glutamicum encoded by
the qsuB gene is related to the previously described QuiC1 protein (39.9% identity) from …
the qsuB gene is related to the previously described QuiC1 protein (39.9% identity) from …