[HTML][HTML] Engineering of thermostable enzymes for industrial applications

F Rigoldi, S Donini, A Redaelli, E Parisini… - APL …, 2018 - pubs.aip.org
The catalytic properties of some selected enzymes have long been exploited to carry out
efficient and cost-effective bioconversions in a multitude of research and industrial sectors …

High-Throughput Screening Techniques for the Selection of Thermostable Enzymes

L Li, X Liu, Y Bai, B Yao, H Luo, T Tu - Journal of Agricultural and …, 2024 - ACS Publications
The acquisition of a thermostable enzyme is an indispensable prerequisite for its successful
implementation in industrial applications and the development of novel functionalities …

Efficient production of protocatechuic acid using systems engineering of Escherichia coli

M Wang, H Wang, C Gao, W Wei, J Liu, X Chen… - Metabolic …, 2024 - Elsevier
Abstract Protocatechuic acid (3, 4-dihydroxybenzoic acid, PCA) is widely used in the
pharmaceuticals, health food, and cosmetics industries owing to its diverse biological …

[HTML][HTML] Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes

L FM Machado, A Currin, N Dixon - Journal of biological engineering, 2019 - Springer
Background Transcription factor-based biosensors are useful tools for the detection of
metabolites and industrially valuable molecules, and present many potential applications in …

Rational engineering of p‐hydroxybenzoate hydroxylase to enable efficient gallic acid synthesis via a novel artificial biosynthetic pathway

Z Chen, X Shen, J Wang, J Wang… - Biotechnology and …, 2017 - Wiley Online Library
Gallic acid (GA) is a naturally occurring phytochemical that has strong antioxidant and
antibacterial activities. It is also used as a potential platform chemical for the synthesis of …

[HTML][HTML] Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

HM Huttanus, EKH Triola… - … in Bioengineering and …, 2023 - frontiersin.org
Targeted mutagenesis of a promoter or gene is essential for attaining new functions in
microbial and protein engineering efforts. In the burgeoning field of synthetic biology …

[HTML][HTML] A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution

RK Jha, JM Bingen, CW Johnson, TL Kern… - Metabolic engineering …, 2018 - Elsevier
Robust fluorescence-based biosensors are emerging as critical tools for high-throughput
strain improvement in synthetic biology. Many biosensors are developed in model …

Proteins from Thermophilic Thermus thermophilus Often Do Not Fold Correctly in a Mesophilic Expression System Such as Escherichia coli

A Kruglikov, Y Wei, X Xia - ACS omega, 2022 - ACS Publications
Majority of protein structure studies use Escherichia coli (E. coli) and other model organisms
as expression systems for other species' genes. However, protein folding depends on …

Sensor-enabled alleviation of product inhibition in chorismate pyruvate-lyase

RK Jha, N Narayanan, N Pandey, JM Bingen… - ACS synthetic …, 2019 - ACS Publications
Product inhibition is a frequent bottleneck in industrial enzymes, and testing mutations to
alleviate product inhibition via traditional methods remains challenging as many variants …

[HTML][HTML] Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

EA Shmonova, OV Voloshina, MV Ovsienko… - PLoS …, 2020 - journals.plos.org
The dehydroshikimate dehydratase (DSD) from Corynebacterium glutamicum encoded by
the qsuB gene is related to the previously described QuiC1 protein (39.9% identity) from …