T-DNA binary vectors and systems

LY Lee, SB Gelvin - Plant physiology, 2008 - academic.oup.com
For more than two decades, scientists have used Agrobacterium-mediated genetic
transformation to generate transgenic plants. Initial technologies to introduce genes of …

Arabidopsis gene knockout: phenotypes wanted

N Bouché, D Bouchez - Current opinion in plant biology, 2001 - Elsevier
Gene knockout is considered to be a major component of the functional genomics toolbox,
and is aimed at revealing the function of genes discovered through large-scale sequencing …

Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana

SJ Clough, AF Bent - The plant journal, 1998 - Wiley Online Library
The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis
thaliana without plant tissue culture or regeneration. In the present study, this method was …

IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth

G Vert, N Grotz, F Dédaldéchamp, F Gaymard… - The plant …, 2002 - academic.oup.com
Plants are the principal source of iron in most diets, yet iron availability often limits plant
growth. In response to iron deficiency, Arabidopsis roots induce the expression of the …

In Planta Agrobacterium-Mediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration

N Bechtold, D Bouchez - Gene transfer to plants, 1995 - Springer
In this chapter, we describe a new protocol for obtaining transgenic Arabidopsis thaliana
plants by a so-called in planta transformation method. Such methods, with no in vitro culture …

The Arabidopsis TT2 Gene Encodes an R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed

N Nesi, C Jond, I Debeaujon, M Caboche… - The Plant …, 2001 - academic.oup.com
In Arabidopsis, proanthocyanidins specifically accumulate in the endothelium during early
seed development. At least three TRANSPARENT TESTA (TT) genes, TT2, TT8, and TTG1 …

[HTML][HTML] Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap

F Gaymard, G Pilot, B Lacombe, D Bouchez… - Cell, 1998 - cell.com
SKOR, a K+ channel identified in Arabidopsis, displays the typical hydrophobic core of the
Shaker channel superfamily, a cyclic nucleotide-binding domain, and an ankyrin domain …

The TT8 Gene Encodes a Basic Helix-Loop-Helix Domain Protein Required for Expression of DFR and BAN Genes in Arabidopsis Siliques

N Nesi, I Debeaujon, C Jond, G Pelletier… - The Plant …, 2000 - academic.oup.com
Abstract The TRANSPARENT TESTA8 (TT8) locus is involved in the regulation of flavonoid
biosynthesis in Arabidopsis. The tt8-3 allele was isolated from a T-DNA–mutagenized …

In planta Agrobacteriummediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration

N Bechtold, G Pelletier - Arabidopsis protocols, 1998 - Springer
Plant genetic transformation was initiated and developed m the 80s thanks to the
convergence of constant progress m the protocol of regeneratton from tissue culture …

The Balance between the MIR164A and CUC2 Genes Controls Leaf Margin Serration in Arabidopsis

K Nikovics, T Blein, A Peaucelle, T Ishida… - The Plant …, 2006 - academic.oup.com
Abstract CUP-SHAPED COTYLEDON1 (CUC1), CUC2, and CUC3 define the boundary
domain around organs in the Arabidopsis thaliana meristem. CUC1 and CUC2 transcripts …