Considerations for the use of phage therapy in clinical practice

GA Suh, TP Lodise, PD Tamma… - Antimicrobial agents …, 2022 - Am Soc Microbiol
Increasing antimicrobial resistance and medical device-related infections have led to a
renewed interest in phage therapy as an alternative or adjunct to conventional …

Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and …

I Michael-Kordatou, P Karaolia… - Journal of environmental …, 2020 - Elsevier
COVID-19 is an ongoing global pandemic caused by severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). As of July 29 th 2020, more than 16, 6 million cases have …

Advances in chemical and biological methods to identify microorganisms—from past to present

R Franco-Duarte, L Černáková, S Kadam, K S. Kaushik… - Microorganisms, 2019 - mdpi.com
Fast detection and identification of microorganisms is a challenging and significant feature
from industry to medicine. Standard approaches are known to be very time-consuming and …

PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

M Sidstedt, P Rådström, J Hedman - Analytical and bioanalytical chemistry, 2020 - Springer
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

PCR inhibitors–occurrence, properties and removal

C Schrader, A Schielke, L Ellerbroek… - Journal of applied …, 2012 - academic.oup.com
The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

Droplet microfluidics for high-throughput biological assays

MT Guo, A Rotem, JA Heyman, DA Weitz - Lab on a Chip, 2012 - pubs.rsc.org
Droplet microfluidics offers significant advantages for performing high-throughput screens
and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to …

Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

MD Parkins, BE Lee, N Acosta, M Bautista… - Clinical Microbiology …, 2024 - Am Soc Microbiol
Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context
of the coronavirus disease 2019 (COVID-19) pandemic. The power and potential of this …

Quantification of mRNA using real-time RT-PCR

T Nolan, RE Hands, SA Bustin - Nature protocols, 2006 - nature.com
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the
evident requirement for quantitative data analysis in molecular medicine, biotechnology …

Impact of gastrointestinal parasitic nematodes of sheep, and the role of advanced molecular tools for exploring epidemiology and drug resistance-an Australian …

F Roeber, AR Jex, RB Gasser - Parasites & vectors, 2013 - Springer
Parasitic nematodes (roundworms) of small ruminants and other livestock have major
economic impacts worldwide. Despite the impact of the diseases caused by these …