Optimising the function of a protein of length N amino acids by directed evolution involves
navigating a 'search space'of possible sequences of some 20 N. Optimising the expression …

Whole-cell biosensors are emerging as promising tools for monitoring environmental
pollutants such as heavy metals. These sensors constitute a genetic circuit comprising a …

The copy number of genes in chromosomes can be modified by chromosomal integration to
construct efficient microbial cell factories but the resulting genetic systems are prone to …

Riboswitches are noncoding RNA switches that are largely utilized in bacteria and play a
significant role in synthetic biology. Nonetheless, their natural counterparts possess lengthy …

The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the
transcription. However, leaking transcription is common due to varied terminator strength …

Although quorum sensing (QS) promoters that can autonomously activate gene expression
have been identified and engineered in Bacillus subtilis, researchers focus on quantifying …

Halomonas bluephagenesis TD01 is one of the ideal chassis for low-cost industrial
production based on “Next Generation Industrial Biotechnology,” yet the limited genetically …

The extracellular substrate-binding proteins (SBPs) of ATP-binding cassette (ABC) importers
tend to be expressed in excess relative to their cognate translocators, but how the …

The primary function of terminators is to terminate transcription in gene expression. Although
some studies have suggested that terminators also contribute positively to upstream gene …

Transcriptional terminators are key players in the flow of genetic information, but are often
overlooked in circuit design. In this work, we used the Standard European Vector …