Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at …

I da Cruz, R Rodríguez-Casuriaga, FF Santiñaque… - BMC genomics, 2016 - Springer
Background Spermatogenesis is a complex differentiation process that involves the
successive and simultaneous execution of three different gene expression programs: mitotic …

Optimized flow cytometry isolation of murine spermatocytes

V Gaysinskaya, IY Soh, GW van der Heijden… - Cytometry Part …, 2014 - Wiley Online Library
Meiotic prophase I (MPI), is an initial stage of meiosis characterized by intricate homologous
chromosome interactions, synapsis, and DNA recombination. These processes depend on …

[HTML][HTML] Contributions of flow cytometry to the molecular study of spermatogenesis in mammals

R Rodríguez-Casuriaga, A Geisinger - International Journal of Molecular …, 2021 - mdpi.com
Mammalian testes are very heterogeneous organs, with a high number of different cell types.
Testicular heterogeneity, together with the lack of reliable in vitro culture systems of …

Revealing stage-specific expression patterns of long noncoding RNAs along mouse spermatogenesis

MF Trovero, R Rodríguez-Casuriaga, C Romeo… - RNA biology, 2020 - Taylor & Francis
The discovery of a large number of long noncoding RNAs (lncRNAs), and the finding that
they may play key roles in different biological processes, have started to provide a new …

Induction of spermatogenesis after stem cell therapy of azoospermic guinea pigs

M Hajihoseini, A Vahdati, S Ebrahim Hosseini… - Veterinarski …, 2017 - hrcak.srce.hr
Sažetak Bone marrow-derived mesenchymal stem cells (BM-MSCs) provide a large
quantitative alternative source for regenerative medicine. This study was undertaken to …

Flow cytometry of murine spermatocytes

V Gaysinskaya, A Bortvin - Current protocols in cytometry, 2015 - Wiley Online Library
Protocols for purification of murine male germ cells by FACS based on Hoechst 33342
(Ho342) dye staining have been reported and optimized. However, the protocols are often …

Comparative proteomic analysis identifies differentially expressed proteins associated with meiotic arrest in cattle‐yak hybrids

SX Wu, R Wan, GW Wang, YW Zhang, QE Yang - Proteomics, 2023 - Wiley Online Library
Cattle‐yak, the interspecific hybrid between yak and taurine cattle, exhibits male‐specific
sterility. Massive loss of spermatogenic cells, especially spermatocytes, results in …

Simple and efficient technique for the preparation of testicular cell suspensions

R Rodríguez-Casuriaga, GA Folle… - Journal of …, 2013 - pmc.ncbi.nlm.nih.gov
Mammalian testes are very complex organs that contain over 30 different cell types,
including somatic testicular cells and different stages of germline cells. This heterogeneity is …

A standardized approach for multispecies purification of mammalian male germ cells by mechanical tissue dissociation and flow cytometry

AC Lima, M Jung, J Rusch, A Usmani… - Journal of …, 2017 - pmc.ncbi.nlm.nih.gov
Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate
enriched populations of mammalian testicular germ cells. Currently, it allows the …

[HTML][HTML] Rapid preparation of rodent testicular cell suspensions and spermatogenic stages purification by flow cytometry using a novel blue-laser-excitable vital dye

R Rodríguez-Casuriaga, FF Santiñaque, GA Folle… - MethodsX, 2014 - Elsevier
Availability of purified or highly enriched fractions representing the various spermatogenic
stages is a usual requirement to study mammalian spermatogenesis at the molecular level …