In addition to light and water, CO 2 and mineral elements are required for plant growth and
development. Among these factors, nitrogen is critical, since it is needed to synthesize …

Saccharomyces cerevisiae is an important industrial cell factory and an attractive
experimental model for evaluating novel metabolic engineering strategies. Many current and …

Chemical modification of transcripts with 5′ caps occurs in all organisms. Here, we report a
systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an …

Quantitative metabolomics is under intense development, and no commonly accepted
standard analytical technique has emerged, yet. The employed analytical methods were …

Sugarcane ethanol fermentation represents a simple microbial community dominated by S.
cerevisiae and co-occurring bacteria with a clearly defined functionality. In this study, we …

The SNF1 protein kinase complex plays an essential role in regulating gene expression in
response to the level of extracellular glucose in budding yeast. SNF1 shares structural and …

Bacterial and eukaryotic nuclear RNA polymerases (RNAPs) cap RNA with the oxidized and
reduced forms of the metabolic effector nicotinamide adenine dinucleotide, NAD+ and …

Quantitative metabolomics of microbial cultures requires well-designed sampling and
quenching procedures. We successfully developed and applied a differential method to …

Cytosolic acetyl-coenzyme A is a precursor for many biotechnologically relevant compounds
produced by Saccharomyces cerevisiae. In this yeast, cytosolic acetyl-CoA synthesis and …

A decade ago, a team of biochemists including two of us, modeled yeast glycolysis and
showed that one of the most studied biochemical pathways could not be quite understood in …