[HTML][HTML] Emergent molecular techniques applied to the detection of porcine viruses

EA Flores-Contreras, JA Carrasco-González… - Veterinary …, 2023 - mdpi.com
Simple Summary In this review, we examine the application of different molecular diagnostic
techniques in the pig industry; qPCR, isothermal methods, and novel techniques such as …

Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues

S Cavalera, B Colitti, GM De Mia, F Feliziani… - Talanta, 2023 - Elsevier
African swine fever (ASF) is a severe haemorrhagic infectious disease affecting suids, thus
representing a great economic concern. Considering the importance of the early diagnosis …

Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless …

Y Wang, H Chen, H Wei, Z Rong, S Wang - Lab on a Chip, 2022 - pubs.rsc.org
Several virulent variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
have emerged along with the spread of this virus throughout the population. Some variants …

Filtration-assisted magnetofluidic cartridge platform for HIV RNA detection from blood

AY Trick, HT Ngo, AH Nambiar, MM Morakis, FE Chen… - Lab on a Chip, 2022 - pubs.rsc.org
The ability to detect and quantify HIV RNA in blood is essential to sensitive detection of
infections and monitoring viremia throughout treatment. Current options for point-of-care HIV …

Mkit: A mobile nucleic acid assay based on a chitosan-modified minimalistic microfluidic chip (CM3-chip) and smartphone

K Yang, J Pan, G Deng, C Hua, C Zhu, Y Liu… - Analytica Chimica Acta, 2023 - Elsevier
Mobile sensing enabled by MS 2 technology, which integrates microfluidic and smartphone
components, has seen many applications in recent years. In this direction, we developed an …

Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification

FE Chen, AY Trick, AC Hasnain, K Hsieh… - Analytical …, 2022 - ACS Publications
Polymerase chain reaction (PCR)-based diagnostic testing is the gold standard method for
pathogen identification (ID) with recent developments enabling automated PCR tests for …

Non-nucleic acid extraction and ultra-sensitive detection of African swine fever virus via CRISPR/Cas12a

G Cao, Y Xiong, F Nie, X Chen, L Peng, Y Li… - Applied Microbiology …, 2022 - Springer
Early diagnosis of the African swine fever virus (ASFV) is the main preventive measure for
ASFV. Here, we developed a fluorescent biosensor and lateral flow assay (LFA) strip based …

[HTML][HTML] Evaluation of a lateral flow assay for rapid detection of African swine fever virus in multiple sample types

C Onyilagha, K Nguyen, PD Luka, U Hussaini… - Pathogens, 2022 - mdpi.com
Antibody-based lateral flow assay (LFA) is a quick and inexpensive tool used to detect
pathogens in field samples, especially in hard-to-reach remote areas that may have limited …

Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations

FE Chen, J Wang, AH Nambiar, J Hardick… - ACS …, 2023 - ACS Publications
Mycoplasma genitalium (MG) is an emerging sexually transmitted bacterium. Due to its
fastidious and slow-growing nature, MG is difficult to detect through culture-based …

A method and optical references for compensating signal drift in a fluorescent microarray reader

G Pichayawaytin, A Somboonkaew, R Jintamethasawat… - Measurement, 2024 - Elsevier
A point-of-need fluorescent microarray assay is a promising tool for on-site high-throughput
and multiplex detection. However, its low-cost and portable read-out system, based on an …