Evaluating cell metabolism through autofluorescence imaging of NAD (P) H and FAD
OI Kolenc, KP Quinn - Antioxidants & redox signaling, 2019 - liebertpub.com
Significance: Optical imaging using the endogenous fluorescence of metabolic cofactors has
enabled nondestructive examination of dynamic changes in cell and tissue function both in …
enabled nondestructive examination of dynamic changes in cell and tissue function both in …
FLIM as a promising tool for cancer diagnosis and treatment monitoring
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …
Protein-bound NAD (P) H lifetime is sensitive to multiple fates of glucose carbon
Abstract While NAD (P) H fluorescence lifetime imaging (FLIM) can detect changes in flux
through the TCA cycle and electron transport chain (ETC), it remains unclear whether NAD …
through the TCA cycle and electron transport chain (ETC), it remains unclear whether NAD …
DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates
R Glazier, JM Brockman, E Bartle… - Nature …, 2019 - nature.com
Podosomes are ubiquitous cellular structures important to diverse processes including cell
invasion, migration, bone resorption, and immune surveillance. Structurally, podosomes …
invasion, migration, bone resorption, and immune surveillance. Structurally, podosomes …
Epithelial layer unjamming shifts energy metabolism toward glycolysis
SJ DeCamp, VMK Tsuda, J Ferruzzi, SA Koehler… - Scientific Reports, 2020 - nature.com
In development of an embryo, healing of a wound, or progression of a carcinoma, a requisite
event is collective epithelial cellular migration. For example, cells at the advancing front of a …
event is collective epithelial cellular migration. For example, cells at the advancing front of a …
Fluorescence intensity and lifetime redox ratios detect metabolic perturbations in T cells
The auto-fluorescent coenzymes reduced nicotinamide dinucleotide (NADH) and oxidized
flavin adenine dinucleotide (FAD) allow label-free detection of cellular metabolism. The …
flavin adenine dinucleotide (FAD) allow label-free detection of cellular metabolism. The …
Separate lifetime signatures of macaque S cones, M/L cones, and rods observed with adaptive optics fluorescence lifetime ophthalmoscopy
In the retina, several molecules involved in metabolism, the visual cycle, and other roles
exhibit intrinsic fluorescence. The overall properties of retinal fluorescence depend on …
exhibit intrinsic fluorescence. The overall properties of retinal fluorescence depend on …
Single-photon peak event detection (SPEED): a computational method for fast photon counting in fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) characterizes samples by examining the
temporal properties of fluorescence emission, providing useful contrast within samples …
temporal properties of fluorescence emission, providing useful contrast within samples …
Distance dependence of plasmon-enhanced fluorescence and delayed luminescence of molecular planar nanostructures
D Temirbayeva, N Ibrayev, M Kucherenko - Journal of Luminescence, 2022 - Elsevier
The distance dependence of the plasmon-accelerated decay of excited singlet and triplet
states of Eosin molecules was studied. Layered films of dye were prepared using the …
states of Eosin molecules was studied. Layered films of dye were prepared using the …
Label-free metabolic imaging in vivo by two-photon fluorescence lifetime endomicroscopy
NADH intensity and fluorescence lifetime characteristics have proved valuable intrinsic
biomarkers for profiling the cellular metabolic status of living biological tissues. To fully …
biomarkers for profiling the cellular metabolic status of living biological tissues. To fully …