New tools for recombinant protein production in Escherichia coli: A 5‐year update

GL Rosano, ES Morales, EA Ceccarelli - Protein science, 2019 - Wiley Online Library
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic
agents. Escherichia coli is the host of choice for recombinant protein production given its fast …

Strategies for enhancing microbial production of 2′-fucosyllactose, the most abundant human milk oligosaccharide

Y Liu, Y Zhu, H Wang, L Wan, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …

Metabolic engineering of Escherichia coli for the production of 2′-fucosyllactose and 3-fucosyllactose through modular pathway enhancement

D Huang, K Yang, J Liu, Y Xu, Y Wang, R Wang… - Metabolic …, 2017 - Elsevier
Abstract Fucosyllactoses, including 2′-fucosyllactose (2′-FL) and 3-fucosyllactose (3-FL),
are important oligosaccharides in human milk that are commonly used as nutritional …

Application of milk permeate as an inducer for the production of microbial recombinant lipolytic enzymes

A Greicius, T Baliutavicius, E Lastauskiene… - Fermentation, 2022 - mdpi.com
Recombinantly produced enzymes are applied in many fields, ranging from medicine to food
and nutrition, production of detergents, textile, leather, paper, pulp, and plastics. Thus, the …

Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization

J Lu, J Tang, Y Liu, X Zhu, T Zhang, X Zhang - Applied microbiology and …, 2012 - Springer
Phosphoenolpyruvate (PEP) is an important precursor for anaerobic production of succinate
and malate. Although inactivating PEP/carbohydrate phosphotransferase systems (PTS) …

Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star (DE3) by modulation of lactose metabolism and fucosyltransferase

YW Chin, JY Kim, WH Lee, JH Seo - Journal of Biotechnology, 2015 - Elsevier
Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk,
which is involved in many biological functions for human health. To date, most microbial …

Acetate metabolism regulation in Escherichia coli: carbon overflow, pathogenicity, and beyond

V Bernal, S Castaño-Cerezo, M Cánovas - Applied microbiology and …, 2016 - Springer
Acetate is ubiquitously found in natural environments. Its availability in the gut is high as a
result of the fermentation of nutrients, and although it is rapidly absorbed by intestinal …

Metabolic responses to recombinant bioprocesses in Escherichia coli

S Carneiro, EC Ferreira, I Rocha - Journal of biotechnology, 2013 - Elsevier
Escherichia coli has been widely used for the production of recombinant proteins. However,
the unbalances between host metabolism and recombinant biosynthesis continue to hamper …

Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct

D Scheps, S Honda Malca, SM Richter… - Microbial …, 2013 - Wiley Online Library
A bacterial P 450 monooxygenase‐based whole cell biocatalyst using Escherichia coli has
been applied in the production of ω‐hydroxy dodecanoic acid from dodecanoic acid (C12 …

Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains

G Lozano Terol, J Gallego-Jara, RA Sola Martínez… - Microbial cell …, 2019 - Springer
Background Escherichia coli (E. coli) is a bacteria that is widely employed in many industries
for the production of high interest bio-products such as recombinant proteins. Nevertheless …