Shotgun metagenomics, from sampling to analysis

C Quince, AW Walker, JT Simpson, NJ Loman… - Nature …, 2017 - nature.com
Diverse microbial communities of bacteria, archaea, viruses and single-celled eukaryotes
have crucial roles in the environment and in human health. However, microbes are …

Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review

H Frickmann, AE Zautner, A Moter… - Critical reviews in …, 2017 - Taylor & Francis
Early identification of microbial pathogens is essential for rational and conservative antibiotic
use especially in the case of known regional resistance patterns. Here, we describe …

Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage

MF Haroon, S Hu, Y Shi, M Imelfort, J Keller… - Nature, 2013 - nature.com
Anaerobic oxidation of methane (AOM) is critical for controlling the flux of methane from
anoxic environments. AOM coupled to iron, manganese and sulphate reduction have been …

The Fibrobacteres: an Important Phylum of Cellulose-Degrading Bacteria

E Ransom-Jones, DL Jones, AJ McCarthy… - Microbial ecology, 2012 - Springer
The phylum Fibrobacteres currently comprises one formal genus, Fibrobacter, and two
cultured species, Fibrobacter succinogenes and Fibrobacter intestinalis, that are recognised …

Visualizing in situ translational activity for identifying and sorting slow-growing archaeal− bacterial consortia

R Hatzenpichler, SA Connon… - Proceedings of the …, 2016 - National Acad Sciences
To understand the biogeochemical roles of microorganisms in the environment, it is
important to determine when and under which conditions they are metabolically active …

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

G Batani, K Bayer, J Böge, U Hentschel, T Thomas - Scientific reports, 2019 - nature.com
Despite the development of several cultivation methods, the rate of discovery of
microorganisms that are yet-to-be cultivated outpaces the rate of isolating and cultivating …

In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry

R Hatzenpichler, S Scheller… - Environmental …, 2014 - Wiley Online Library
Here we describe the application of a new click chemistry method for fluorescent tracking of
protein synthesis in individual microorganisms within environmental samples. This …

Antibody engineering for pursuing a healthier future

AFUH Saeed, R Wang, S Ling, S Wang - Frontiers in microbiology, 2017 - frontiersin.org
Since the development of antibody-production techniques, a number of immunoglobulins
have been developed on a large scale using conventional methods. Hybridoma technology …

The trajectory of microbial single-cell sequencing

T Woyke, DFR Doud, F Schulz - Nature Methods, 2017 - nature.com
Over the past decade, it has become nearly routine to sequence genomes of individual
microbial cells directly isolated from environmental samples ranging from deep-sea …

Unravelling the identity, metabolic potential and global biogeography of the atmospheric methane‐oxidizing upland soil cluster α

J Pratscher, J Vollmers, S Wiegand… - Environmental …, 2018 - Wiley Online Library
Understanding of global methane sources and sinks is a prerequisite for the design of
strategies to counteract global warming. Microbial methane oxidation in soils represents the …