Ever-changing landscapes: transcriptional enhancers in development and evolution

HK Long, SL Prescott, J Wysocka - Cell, 2016 - cell.com
A class of cis-regulatory elements, called enhancers, play a central role in orchestrating
spatiotemporally precise gene-expression programs during development. Consequently …

Transcription factor–DNA binding: beyond binding site motifs

S Inukai, KH Kock, ML Bulyk - Current opinion in genetics & development, 2017 - Elsevier
Sequence-specific transcription factors (TFs) regulate gene expression by binding to cis-
regulatory elements in promoter and enhancer DNA. While studies of TF–DNA binding have …

G-quadruplexes are transcription factor binding hubs in human chromatin

J Spiegel, SM Cuesta, S Adhikari, R Hänsel-Hertsch… - Genome biology, 2021 - Springer
Background The binding of transcription factors (TF) to genomic targets is critical in the
regulation of gene expression. Short, double-stranded DNA sequence motifs are routinely …

[HTML][HTML] Structural basis for Cas9 off-target activity

M Pacesa, CH Lin, A Cléry, A Saha, PR Arantes… - Cell, 2022 - cell.com
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …

Homeodomain proteins: an update

TR Bürglin, M Affolter - Chromosoma, 2016 - Springer
Here, we provide an update of our review on homeobox genes that we wrote together with
Walter Gehring in 1994. Since then, comprehensive surveys of homeobox genes have …

Low-affinity binding sites and the transcription factor specificity paradox in eukaryotes

JF Kribelbauer, C Rastogi… - Annual review of cell …, 2019 - annualreviews.org
Eukaryotic transcription factors (TFs) from the same structural family tend to bind similar DNA
sequences, despite the ability of these TFs to execute distinct functions in vivo. The cell …

Quantum annealing versus classical machine learning applied to a simplified computational biology problem

RY Li, R Di Felice, R Rohs, DA Lidar - NPJ quantum information, 2018 - nature.com
Transcription factors regulate gene expression, but how these proteins recognize and
specifically bind to their DNA targets is still debated. Machine learning models are effective …

Order through disorder: The role of intrinsically disordered regions in transcription factor binding specificity

S Brodsky, T Jana, N Barkai - Current opinion in structural biology, 2021 - Elsevier
Transcription factors (TFs) must bind at specific genomic locations to accurately regulate
gene expression. The ability of TFs to recognize specific DNA sequence motifs arises from …

Predicting DNA structure using a deep learning method

J Li, TP Chiu, R Rohs - Nature Communications, 2024 - nature.com
Understanding the mechanisms of protein-DNA binding is critical in comprehending gene
regulation. Three-dimensional DNA structure, also described as DNA shape, plays a key …

ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo

GE Zentner, S Kasinathan, B Xin, R Rohs… - Nature …, 2015 - nature.com
Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal
nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo …