Monocarboxylates such as lactate and pyruvate play a central role in cellular metabolism
and metabolic communication between tissues. Essential to these roles is their rapid …

Dysregulated metabolism is one of the hallmarks of cancer. Under normal physiological
conditions, ATP is primarily generated by oxidative phosphorylation. Cancers commonly …

The effects of mutation of key active-site residues (Arg-47, Tyr-51, Phe-42 and Phe-87) in
Bacillus megaterium flavocytochrome P450 BM3 were investigated. Kinetic studies on the …

Measurement of monocarboxylate transport kinetics in a range of cell types has provided
strong circumstantial evidence for a family of monocarboxylate transporters (MCTs). Two …

The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.
1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli …

Analysis of the mechanism and structure of lactate dehydrogenases is summarized in a map
of the catalytic pathway. Chemical probes, single tryptophan residues inserted at specific …

Monocarboxylate transporters MCT1-MCT4 require basigin (CD147) or embigin (gp70),
ancillary proteins with a glutamate residue in their single transmembrane (TM) domain, for …

Malate and lactate dehydrogenases (MDH and LDH) are homologous, core metabolic
enzymes that share a fold and catalytic mechanism yet possess strict specificity for their …

Classic techniques for simulating molecular motion, such as the Monte Carlo and molecular
dynamics methods, generate individual motion pathways one at a time and spend most of …

Abstract Background: L (+)-Lactate dehydrogenase (LDH) catalyzes the last step in
anaerobic glycolysis, the conversion of pyruvate to lactate, with the concomitant oxidation of …